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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-TDP43 antibody [EPR5810] (ab193842)

Reviews (1) Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TDP43 antibody [EPR5810] (ab193842)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TDP43 antibody [EPR5810] (ab193842)
  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TDP43 antibody [EPR5810] (ab193842)
  • Alexa Fluor® 488 Anti-TDP43 antibody [EPR5810] (ab193842)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR5810] to TDP43
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

APC Carrier Free HRP PE Unconjugated

Overview

  • Product name

    Alexa Fluor® 488 Anti-TDP43 antibody [EPR5810]
    See all TDP43 primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR5810] to TDP43

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Hek293 and HAP1-TARDBP cells. Flow Cyt (intra): Hek293 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab193842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 1 µg/ml.

This product gave a positive signal in Hek293 cells fixed with 100% methanol (5 min). This antibody is suitable to detect TDP43 using MeOH fixation in ICC.We have compared methanol and paraformaldehyde (PFA) fixation methods with this product and recommend to use methanol only.
Flow Cyt (Intra)
1/500.
Notes
ICC/IF
Use a concentration of 1 µg/ml.

This product gave a positive signal in Hek293 cells fixed with 100% methanol (5 min). This antibody is suitable to detect TDP43 using MeOH fixation in ICC.We have compared methanol and paraformaldehyde (PFA) fixation methods with this product and recommend to use methanol only.
Flow Cyt (Intra)
1/500.

Target

  • Function

    DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.

  • Tissue specificity

    Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.

  • Involvement in disease

    Defects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.

  • Sequence similarities

    Contains 2 RRM (RNA recognition motif) domains.

  • Domain

    The RRM domains can bind to both DNA and RNA.

  • Post-translational
    modifications

    Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
    Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
    Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.

  • Cellular localization

    Nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.
  • Information by UniProt

  • Database links

    • Alternative names

      • ALS10 antibody
      • OTTHUMP00000002171 antibody
      • OTTHUMP00000002172 antibody
      • OTTHUMP00000002173 antibody
      • TADBP_HUMAN antibody
      • TAR DNA binding protein 43 antibody
      • TAR DNA binding protein antibody
      • TAR DNA-binding protein 43 antibody
      • TARDBP antibody
      • TDP 43 antibody
      • TDP-43 antibody
      • TDP43 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TDP43 antibody [EPR5810] (ab193842)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TDP43 antibody [EPR5810] (ab193842)

      ab193842 staining TDP43 in wild-type HAP1 cells (top panel) and TDP43 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab193842 at 1μg/ml (shown in green) and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This antibody is not suitable to detect TDP43 using PFA fixation in ICC.

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TDP43 antibody [EPR5810] (ab193842)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TDP43 antibody [EPR5810] (ab193842)

      ab193842 staining TDP43 in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab193842 at a 1/250 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This antibody is not suitable to detect TDP43 using PFA fixation in ICC.

    • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TDP43 antibody [EPR5810] (ab193842)
      Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TDP43 antibody [EPR5810] (ab193842)
      Overlay histogram showing HEK293 cells stained with ab193842 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab193842, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Alexa Fluor® 488 Anti-TDP43 antibody [EPR5810] (ab193842)
      Alexa Fluor® 488 Anti-TDP43 antibody [EPR5810] (ab193842)

    References (1)

    Publishing research using ab193842? Please let us know so that we can cite the reference in this datasheet.

    ab193842 has been referenced in 1 publication.

    • Rashid R  et al. Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer. Sci Data 6:323 (2019). beta actin ; Human . PubMed: 31848351

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TDP43 antibody [EPR5810] (Alexa Fluor® 488)

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Chinese Hamster Cell (Chinese Hamster Ovary (CHO) cells)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    Chinese Hamster Ovary (CHO) cells
    Blocking step
    TBS with 1% BSA, 0.1% Tween 20 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 30 2015

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