Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)

Submit a review Submit a question
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)
  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)
  • Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [106-6] to TMEM119
  • Suitable for: Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free PE Unconjugated

Overview

  • Product name

    Alexa Fluor® 488 Anti-TMEM119 antibody [106-6]
    See all TMEM119 primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [106-6] to TMEM119

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment (MBP-tag) within Mouse TMEM119 aa 1-100 (extracellular). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q8R138

  • Positive control

    • Flow Cyt (Intra): HEK-293T transfected with Myc-His tagged TMEM119 expression vector; CD11b+ brain cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    106-6

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab225497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.

The cellular localisation of this product has been verified in ICC/IF.

This antibody may be used at 10 µg/mL.
Notes
Flow Cyt (Intra)
1/500.

The cellular localisation of this product has been verified in ICC/IF.

This antibody may be used at 10 µg/mL.

Target

Images

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)
    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)

    Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225497 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Alexa Fluor® 488 ab199091 (left). Cells were surface-stained with ab225497, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)
    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)This image is courtesy of an anonymous collaborator.

    Flow cytometric analysis of wild type (WT) versus Tmem119 knockout (KO) CD11b+ cells labeling TMEM119 with ab225497 at 10 μg/mL (green) or the same clone unconjugated (ab210405) at 1 μg/mL with an Alexa 488 secondary antibody (purple), compared with TMEM119 KO primary mouse brain cells stained with ab210405 at 1 μg/mL (black) and ab225497 at 10 μg/mL (grey). Goat anti-Rabbit IgG (Alexa Fluor®488) at 1:1000 dilution was used as the secondary antibody.

    No signal was detected on the surface of CD11b+ brain cells from TMEM119 KO mouse stained with either the unconjugated ab210405 (black) or conjugated ab225497 antibodies (grey); whereas in wildtype CD11b+ brain cells, cell surface staining was observed (green, Alexa conjugated ab225497; purple, unconjugated ab210405 plus secondary antibody).

  • Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)
    Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab225497? Please let us know so that we can cite the reference in this datasheet.

ab225497 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab225497.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com