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RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-TSG101 antibody [EPR7130(B)] (ab207663)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TSG101 antibody [EPR7130(B)] (ab207663)
  • Alexa Fluor® 488 Anti-TSG101 antibody [EPR7130(B)] (ab207663)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR7130(B)] to TSG101
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free PE Unconjugated

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Overview

  • Product name

    Alexa Fluor® 488 Anti-TSG101 antibody [EPR7130(B)]
    See all TSG101 primary antibodies
  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR7130(B)] to TSG101
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Jurkat cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7130(B)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Other
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Differentiation
    • Cancer
    • Cell cycle
    • Cell differentiation
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Other

Associated products

  • Alternative Versions

    • Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    • Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567)
    • Alexa Fluor® 647 Anti-TSG101 antibody [EPR7130(B)] (ab207664)
    • PE Anti-TSG101 antibody [EPR7130(B)] (ab209927)
    • Alexa Fluor® 555 Anti-TSG101 antibody [EPR7130(B)] (ab214404)
    • Anti-TSG101 antibody [EPR7130(B)] - Low endotoxin, Azide free (ab216447)
  • Isotype control

    • Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse brain tissue lysate - total protein (0 days) (ab7188)
    • Mouse brain tissue lysate - total protein (14 days) (ab7189)
    • Jurkat whole cell lysate (ab7899)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab207663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min).

Notes
ICC/IF
1/100.

This product gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min).

Target

  • Function

    Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
  • Tissue specificity

    Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
    Contains 1 SB (steadiness box) domain.
    Contains 1 UEV (ubiquitin E2 variant) domain.
  • Domain

    The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
    The coiled coil domain may interact with stathmin.
    The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.
  • Post-translational
    modifications

    Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
  • Cellular localization

    Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
  • Target information above from: UniProt accession Q99816 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7251 Human
    • Entrez Gene: 22088 Mouse
    • Entrez Gene: 292925 Rat
    • Omim: 601387 Human
    • SwissProt: Q99816 Human
    • SwissProt: Q61187 Mouse
    • SwissProt: Q6IRE4 Rat
    • Unigene: 523512 Human
    • Unigene: 241334 Mouse
    • Unigene: 7410 Rat
    see all
  • Alternative names

    • ESCRT I complex subunit TSG101 antibody
    • ESCRT-I complex subunit TSG101 antibody
    • TS101_HUMAN antibody
    • TSG 10 antibody
    • TSG 101 antibody
    • TSG10 antibody
    • Tsg101 antibody
    • Tumor susceptibility 101 antibody
    • Tumor susceptibility gene 10 antibody
    • Tumor susceptibility gene 101 antibody
    • Tumor susceptibility gene 101 protein antibody
    • Tumor susceptibility protein antibody
    • Tumor susceptibility protein isoform 3 antibody
    • VPS 23 antibody
    • VPS23 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TSG101 antibody [EPR7130(B)] (ab207663)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TSG101 antibody [EPR7130(B)] (ab207663)

    ab207663 staining TSG101 in Jurkat cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207663 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-TSG101 antibody [EPR7130(B)] (ab207663)
    Alexa Fluor® 488 Anti-TSG101 antibody [EPR7130(B)] (ab207663)

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab207663? Please let us know so that we can cite the reference in this datasheet.

ab207663 has been referenced in 1 publication.

  • Xiao B  et al. Endothelial cell-derived exosomes protect SH-SY5Y nerve cells against ischemia/reperfusion injury. Int J Mol Med 40:1201-1209 (2017). PubMed: 28849073

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