Recombinant Alexa Fluor® 488 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab302827)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Tyrosine Hydroxylase with ab302827 at 1/50 dilution (10.0 µg/ml). Dendritic staining on the striatum of mouse cerebrum is observed. The section was incubated with ab302827 for 60 mins at room temperature (shown in green). Negative control: Primary diluent was used instead of primary antibody. The section was then mounted using Fluoromount^®. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins was used. 

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Tyrosine Hydroxylase with ab302827 at 1/50 dilution (10.0 µg/ml). Dendritic staining on the striatum of rat cerebrum is observed. The section was incubated with ab302827 for 60 mins at room temperature (shown in green). Negative control: Primary diluent was used instead of primary antibody. The section was then mounted using Fluoromount^®. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins was used.

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labeling Tyrosine Hydroxylase with ab302827 at 1/50 dilution (1 µg) (Red), compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor^® 488) (ab199091) (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).