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RecombinantRabMAb

Recombinant Alexa Fluor® 555 Anti-BDNF antibody [EPR1292] (ab305366)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-BDNF antibody [EPR1292] (ab305366)
  • Flow Cytometry (Intracellular) - Alexa Fluor® 555 Anti-BDNF antibody [EPR1292] (ab305366)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 555 Rabbit monoclonal [EPR1292] to BDNF
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free Unconjugated

Overview

  • Product name

    Alexa Fluor® 555 Anti-BDNF antibody [EPR1292]
    See all BDNF primary antibodies

  • Description

    Alexa Fluor® 555 Rabbit monoclonal [EPR1292] to BDNF

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm

  • Specificity

    This product may cross react with the following family members: NGF beta, neurotrophin 3, neurotrophin 4.

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra)more details
    Unsuitable for: IHC-Fr

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells. Flow Cyt (Intra): HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab305366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/50.
Flow Cyt (Intra)
1/500.
Notes
ICC/IF
1/50.
Flow Cyt (Intra)
1/500.
Application notes
Is unsuitable for IHC-Fr.

Target

  • Function

    During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

  • Tissue specificity

    Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.

  • Involvement in disease

    Bulimia nervosa 2
    Congenital central hypoventilation syndrome

  • Sequence similarities

    Belongs to the NGF-beta family.

  • Post-translational
    modifications

    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Abrineurin antibody
      • ANON2 antibody
      • BDNF antibody
      • BDNF_HUMAN antibody
      • Brain Derived Neurotrophic Factor antibody
      • Brain-derived neurotrophic factor antibody
      • BULN2 antibody
      • MGC34632 antibody
      • Neurotrophin antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-BDNF antibody [EPR1292] (ab305366)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-BDNF antibody [EPR1292] (ab305366)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling BDNF with ab305366 at 1/50 dilution (10.0 ug/ml) (Red). Confocal image showing co-locolization staining with golgi marker in HeLa cells.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker was used to counterstain tubulin at 1/200 dilution (5 ug/ml) (Green). The nuclear counterstain was DAPI (Blue).

    • Flow Cytometry (Intracellular) - Alexa Fluor® 555 Anti-BDNF antibody [EPR1292] (ab305366)
      Flow Cytometry (Intracellular) - Alexa Fluor® 555 Anti-BDNF antibody [EPR1292] (ab305366)

      Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling BDNF with ab305366 at 1/500 dilution (0.1 ug) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 555) (ab208569) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab305366? Please let us know so that we can cite the reference in this datasheet.

    ab305366 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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