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RecombinantRabMAb

Recombinant Alexa Fluor® 555 Anti-Dopamine Transporter antibody [EPR19695] (ab302551)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Dopamine Transporter antibody [EPR19695] (AB302551)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Dopamine Transporter antibody [EPR19695] (AB302551)
  • Alexa Fluor® 555 Anti-Dopamine Transporter antibody [EPR19695] (AB302551)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 555 Rabbit monoclonal [EPR19695] to Dopamine Transporter
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat
  • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free Unconjugated

Overview

  • Product name

    Alexa Fluor® 555 Anti-Dopamine Transporter antibody [EPR19695]
    See all Dopamine Transporter primary antibodies

  • Description

    Alexa Fluor® 555 Rabbit monoclonal [EPR19695] to Dopamine Transporter

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm

  • Specificity

    AB302551 does not react in frozen section immunohistochemistry with mous and rat species. 

  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse and rat striatum tissue.

  • General notes

     

     

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab302551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for IHC-Fr.

Target

  • Function

    Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.

  • Involvement in disease

    Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI) [MIM:613135]. It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs.

  • Sequence similarities

    Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • DA transporter antibody
      • DAT 1 antibody
      • DAT antibody
      • DAT1 antibody
      • Dopamine transporter 1 antibody
      • Dopamine transporter antibody
      • PKDYS antibody
      • SC6A3_HUMAN antibody
      • SLC6A3 antibody
      • Sodium dependent dopamine transporter antibody
      • Sodium-dependent dopamine transporter antibody
      • Solute carrier family 6 (neurotransmitter transporter dopamine), member 3 antibody
      • Solute carrier family 6 (neurotransmitter transporter), member 3 antibody
      • Solute carrier family 6 member 3 antibody
      • Variable number tandem repeat (VNTR) antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Dopamine Transporter antibody [EPR19695] (AB302551)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Dopamine Transporter antibody [EPR19695] (AB302551)

      Immunohistochemical analysis of paraffin-embedded mouse striatum tissue labeling dopamine transporter with ab302551 at 1/100 dilution (5.0 µg/mL). Positive staining on mouse striatum is observed. The section was incubated with ab30551 for 60 mins at room temperature (shown in green). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The nuclear counterstain was DAPI (Blue). Negative control: Primary diluent was used instead of primary antibody. The section was then mounted using Fluoromount®. 

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins was used. 

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Dopamine Transporter antibody [EPR19695] (AB302551)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Dopamine Transporter antibody [EPR19695] (AB302551)

      Immunohistochemical analysis of paraffin-embedded rat striatum tissue labeling dopamine transporter with ab302551 at 1/100 dilution (5.0 µg/mL). Positive staining on rat striatum is observed. The section was incubated with ab30551 for 60 mins at room temperature (shown in green). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The nuclear counterstain was DAPI (Blue). Negative control: Primary diluent was used instead of primary antibody. The section was then mounted using Fluoromount®. 

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins was used. 

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). 

    • Alexa Fluor® 555 Anti-Dopamine Transporter antibody [EPR19695] (AB302551)
      Alexa Fluor® 555 Anti-Dopamine Transporter antibody [EPR19695] (AB302551)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab302551? Please let us know so that we can cite the reference in this datasheet.

    ab302551 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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