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    products/primary-antibodies/alexa-fluor-555-lc3b-autophagosome-marker-antibody-epr18709-ab307768.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules MT Associated Proteins MAP
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RecombinantRabMAb

Recombinant Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)
  • Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 555 Rabbit monoclonal [EPR18709] to LC3B - Autophagosome Marker
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free Unconjugated

Overview

  • Product name

    Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709]
    See all LC3B primary antibodies
  • Description

    Alexa Fluor® 555 Rabbit monoclonal [EPR18709] to LC3B - Autophagosome Marker
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human cerebrum and glioma tissue. ICC/IF: Chloroquine treated HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68.98% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18709
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MAP
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Cardiovascular
    • Heart
    • Autophagy
    • APG gene products
    • Cancer
    • Signal transduction
    • Autophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • APG gene products
    • Cancer
    • Cell Death
    • Autophagy
    • Signal Transduction
    • Cancer
    • Cell Death
    • Autophagy
    • APG gene products
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-LC3B antibody [EPR18709] - BSA and Azide free (ab221794)
    • Alexa Fluor® 488 Anti-LC3B antibody [EPR18709] - Autophagosome Marker (ab225382)
    • Alexa Fluor® 647 Anti-LC3B antibody [EPR18709] - Autophagosome Marker (ab225383)
  • Related Products

    • Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)
    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab307768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/50.
IHC-P
1/100.
Notes
ICC/IF
1/50.
IHC-P
1/100.

Target

  • Function

    Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • Tissue specificity

    Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
  • Sequence similarities

    Belongs to the MAP1 LC3 family.
  • Post-translational
    modifications

    The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.
  • Cellular localization

    Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Target information above from: UniProt accession Q9GZQ8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 81631 Human
    • Omim: 609604 Human
    • SwissProt: Q9GZQ8 Human
    • Unigene: 356061 Human
    • Alternative names

      • ATG8F antibody
      • Autophagy-related protein LC3 B antibody
      • Autophagy-related ubiquitin-like modifier LC3 B antibody
      • LC3B antibody
      • LC3II antibody
      • MAP1 light chain 3 like protein 2 antibody
      • MAP1 light chain 3-like protein 2 antibody
      • MAP1A/1BLC3 antibody
      • MAP1A/MAP1B LC3 B antibody
      • MAP1A/MAP1B light chain 3 B antibody
      • MAP1ALC3 antibody
      • MAP1LC3B a antibody
      • Map1lc3b antibody
      • Microtubule associated protein 1 light chain 3 beta antibody
      • Microtubule-associated protein 1 light chain 3 beta antibody
      • Microtubule-associated proteins 1A/1B light chain 3B antibody
      • MLP3B_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)

      Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling LC3B with ab307768 at 1/100 dilution (5.0 µg/ml).

      Cytoplasmic staining on human cerebrum.

      The section was incubated with ab307768 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
      Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)

      Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling LC3B with ab307768 at 1/100 dilution (5.0 µg/ml).
      Cytoplasmic staining on human glioma.
      The section was incubated with ab307768 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
      Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)

      Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling LC3B with ab307768 at 1/100 dilution (5.0 µg/ml).

      Negative control: no staining on human stomach.


      The section was incubated with ab307768 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
      Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)

      Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labeling LC3B with ab307768 at 1/50 dilution (10 µg/ml) (Red).

      Confocal image showing increased cytoplasmic staining in HeLa cells treated with Chloroquine (50μM) for 24 hours.

      ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Green). The nuclear counterstain was DAPI (Blue).

    • Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)
      Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] (ab307768)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab307768? Please let us know so that we can cite the reference in this datasheet.

    ab307768 has not yet been referenced specifically in any publications.

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