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    products/primary-antibodies/alexa-fluor-555-runx1--aml1runx3runx2-antibody-epr3099-ab207253.pdf

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RecombinantRabMAb

Recombinant Alexa Fluor® 555 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099] (ab207253)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099] (ab207253)
  • Alexa Fluor® 555 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099] (ab207253)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 555 Rabbit monoclonal [EPR3099] to RUNX1 / AML1+RUNX3+RUNX2
  • Suitable for: ICC/IF
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm

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Overview

  • Product name

    Alexa Fluor® 555 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099]
    See all RUNX1 / AML1+RUNX3+RUNX2 primary antibodies
  • Description

    Alexa Fluor® 555 Rabbit monoclonal [EPR3099] to RUNX1 / AML1+RUNX3+RUNX2
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide within Human RUNX1/ AML1+RUNX3+RUNX2 aa 400 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q01196
    (Peptide available as ab177141)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • ICC/IF: Neuro2a cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3099
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Stem Cells
    • Mesenchymal Stem Cells
    • Osteogenesis
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Stem Cells
    • Hematopoietic Progenitors
    • Hemangioblast
    • Developmental Biology
    • Organogenesis
    • Hematopoietic system development
    • Developmental Biology
    • Organogenesis
    • Skeletal development
    • Bone
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab199221)
    • Alexa Fluor® 647 Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab199493)
    • Alexa Fluor® 594 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099] (ab207251)
    • Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)
    • Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336)
  • Immunizing Peptide (Blocking)

    • RUNX1 / AML1 peptide (ab177141)
  • Isotype control

    • Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab207253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/50.

This product gave a positive signal in Neuro2a cells fixed with 4% formaldehyde (10 min)

Notes
ICC/IF
1/50.

This product gave a positive signal in Neuro2a cells fixed with 4% formaldehyde (10 min)

Target

  • Cellular localization

    RUNX1 / AML1: Nucleus. RUNX3: Nucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm. RUNX2: Nucleus.
  • Database links

    • Entrez Gene: 860 Human
    • Entrez Gene: 861 Human
    • Entrez Gene: 864 Human
    • Entrez Gene: 12393 Mouse
    • Entrez Gene: 12394 Mouse
    • Entrez Gene: 12399 Mouse
    • Entrez Gene: 156726 Rat
    • Entrez Gene: 367218 Rat
    • Entrez Gene: 50662 Rat
    • Omim: 151385 Human
    • Omim: 600210 Human
    • Omim: 600211 Human
    • SwissProt: Q01196 Human
    • SwissProt: Q13761 Human
    • SwissProt: Q13950 Human
    • SwissProt: Q03347 Mouse
    • SwissProt: Q08775 Mouse
    • SwissProt: Q64131 Mouse
    • SwissProt: Q63046 Rat
    • SwissProt: Q9Z2J9 Rat
    • Unigene: 149261 Human
    • Unigene: 170019 Human
    • Unigene: 535845 Human
    • Unigene: 612648 Human
    • Unigene: 378894 Mouse
    • Unigene: 391013 Mouse
    • Unigene: 391017 Mouse
    • Unigene: 4081 Mouse
    • Unigene: 470227 Mouse
    • Unigene: 11201 Rat
    • Unigene: 214214 Rat
    • Unigene: 83672 Rat
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099] (ab207253)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099] (ab207253)

    ab207253 staining RUNX1 / AML1+RUNX3+RUNX2 in Neuro2a cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207253 at a 1/50 dilution (pseudocolored in green) and ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor® 647), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 555 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099] (ab207253)
    Alexa Fluor® 555 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099] (ab207253)

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab207253? Please let us know so that we can cite the reference in this datasheet.

ab207253 has not yet been referenced specifically in any publications.

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