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    products/primary-antibodies/alexa-fluor-555-tyrosine-hydroxylase-antibody-ep1532y-neuronal-marker-ab305365.pdf

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RecombinantRabMAb

Recombinant Alexa Fluor® 555 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab305365)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab305365)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab305365)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 555 Rabbit monoclonal [EP1532Y] to Tyrosine Hydroxylase - Neuronal Marker
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat
  • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 568 Carrier Free HRP Unconjugated

Overview

  • Product name

    Alexa Fluor® 555 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker
    See all Tyrosine Hydroxylase primary antibodies
  • Description

    Alexa Fluor® 555 Rabbit monoclonal [EP1532Y] to Tyrosine Hydroxylase - Neuronal Marker
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt (Intra) or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse and rat cerebrum tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1532Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Dopamine
    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Noradrenaline
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • HRP Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab207673)
    • Anti-Tyrosine Hydroxylase antibody [EP1532Y] - BSA and Azide free (ab220218)
    • Alexa Fluor® 488 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab302827)
    • Alexa Fluor® 568 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab302968)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab305365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt (Intra) or ICC/IF.

Target

  • Function

    Plays an important role in the physiology of adrenergic neurons.
  • Tissue specificity

    Mainly expressed in the brain and adrenal glands.
  • Pathway

    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • Involvement in disease

    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • Sequence similarities

    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Target information above from: UniProt accession P07101 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 21823 Mouse
    • Entrez Gene: 25085 Rat
    • SwissProt: P24529 Mouse
    • SwissProt: P04177 Rat
    • Unigene: 1292 Mouse
    • Unigene: 11082 Rat
    • Alternative names

      • Dystonia 14 antibody
      • DYT14 antibody
      • DYT5b antibody
      • EC 1.14.16.2 antibody
      • OTTHUMP00000011225 antibody
      • OTTHUMP00000011226 antibody
      • ple antibody
      • Protein Pale antibody
      • TH antibody
      • The antibody
      • TY3H_HUMAN antibody
      • TYH antibody
      • Tyrosine 3 hydroxylase antibody
      • Tyrosine 3 monooxygenase antibody
      • Tyrosine 3-hydroxylase antibody
      • Tyrosine 3-monooxygenase antibody
      • Tyrosine hydroxylase antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab305365)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab305365)

      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Tyrosine Hydroxylase with ab305365 at 1/100 dilution (5.0 ug/ml). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab305365 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab305365)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab305365)

      Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Tyrosine Hydroxylase with ab305365 at 1/100 dilution (5.0 ug/ml). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab305365 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab305365? Please let us know so that we can cite the reference in this datasheet.

    ab305365 has not yet been referenced specifically in any publications.

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