Recombinant Alexa Fluor® 568 Anti-beta IV Tubulin antibody [EPR16775] (ab312543)
![Alexa Fluor® 568 Anti-beta IV Tubulin antibody [EPR16775] (ab312543)](https://www.abcam.com/ps/products/312/ab312543/Images/ab312543-1-MTOConjugatedRecombinantAntibodyBenefits.jpg "Alexa Fluor® 568 Anti-beta IV Tubulin antibody [EPR16775] (ab312543)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 568 Rabbit monoclonal [EPR16775] to beta IV Tubulin
- Suitable for: Target binding affinity, Antibody labelling
- Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 568 Anti-beta IV Tubulin antibody [EPR16775]
See all beta IV Tubulin primary antibodies -
Description
Alexa Fluor® 568 Rabbit monoclonal [EPR16775] to beta IV Tubulin -
Host species
Rabbit -
Conjugation
Alexa Fluor® 568. Ex: 578nm, Em: 603nm -
Tested applications
Suitable for: Target binding affinity, Antibody labellingmore details -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for Abtrial: learn more here.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16775 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-beta IV Tubulin antibody [EPR16775] (ab179504)
- Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
- PE Anti-beta IV Tubulin antibody [EPR16775] (ab307180)
- APC Anti-beta IV Tubulin antibody [EPR16775] (ab307181)
- HRP Anti-beta IV Tubulin antibody [EPR16775] (ab307182)
- AP Anti-beta IV Tubulin antibody [EPR16775] (ab308805)
- Alexa Fluor® 594 Anti-beta IV Tubulin antibody [EPR16775] (ab310535)
- Alexa Fluor® 555 Anti-beta IV Tubulin antibody [EPR16775] (ab312066)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab312543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Target binding affinity |
Use at an assay dependent concentration.
|
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| Antibody labelling |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Target binding affinity
Use at an assay dependent concentration. |
|
Antibody labelling
Use at an assay dependent concentration. |
Target
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Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. -
Sequence similarities
Belongs to the tubulin family. -
Domain
The highly acidic C-terminal region may bind cations such as calcium. -
Post-translational
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Alternative names
- Beta 4 antibody
- Beta 4 tubulin antibody
- beta 5 antibody
see all
Images
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Alexa Fluor® 568 Anti-beta IV Tubulin antibody [EPR16775] (ab312543)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab312543 has not yet been referenced specifically in any publications.