Recombinant Alexa Fluor® 568 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab312381)
![Alexa Fluor® 568 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab312381)](https://www.abcam.com/ps/products/312/ab312381/Images/ab312381-1-MTOConjugatedRecombinantAntibodyBenefits.jpg "Alexa Fluor® 568 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab312381)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 568 Rabbit monoclonal [EP265(2)Y] to CD3 zeta
- Suitable for: Target binding affinity, Antibody labelling
- Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 568 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y]
See all CD3 zeta primary antibodies -
Description
Alexa Fluor® 568 Rabbit monoclonal [EP265(2)Y] to CD3 zeta -
Host species
Rabbit -
Conjugation
Alexa Fluor® 568. Ex: 578nm, Em: 603nm -
Tested applications
Suitable for: Target binding affinity, Antibody labellingmore details -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for Abtrial: learn more here.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP265(2)Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)
- PE Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab305800)
- APC Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab305801)
- Alexa Fluor® 488 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab309652)
- Alexa Fluor® 647 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab310016)
- Alexa Fluor® 594 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab310384)
- Alexa Fluor® 555 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab311910)
- Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab68235)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab312381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Target binding affinity |
Use at an assay dependent concentration.
|
|
| Antibody labelling |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Target binding affinity
Use at an assay dependent concentration. |
|
Antibody labelling
Use at an assay dependent concentration. |
Target
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Function
Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. -
Involvement in disease
Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens. -
Sequence similarities
Belongs to the CD3Z/FCER1G family.
Contains 3 ITAM domains. -
Domain
The ITAM domains mediate interaction with SHB. -
Post-translational
modificationsPhosphorylated on Tyr residues after T-cell receptor triggering. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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Alexa Fluor® 568 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab312381)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab312381 has not yet been referenced specifically in any publications.