Recombinant Alexa Fluor® 568 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203445)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203445)](https://www.abcam.com/ps/products/203/ab203445/Images/ab203445-248327-anti-cytokeratin-19-antibody-ep1580y-alexa-fluor-568-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203445)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 568 Rabbit monoclonal [EP1580Y] to Cytokeratin 19
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 568 Anti-Cytokeratin 19 antibody [EP1580Y]
See all Cytokeratin 19 primary antibodies -
Description
Alexa Fluor® 568 Rabbit monoclonal [EP1580Y] to Cytokeratin 19 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 568. Ex: 578nm, Em: 603nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HeLa cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 3.70 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1580Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192643)
- Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192980)
- HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600)
- Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
- Alexa Fluor® 594 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203443)
- Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203444)
- APC Anti-Cytokeratin 19 antibody [EP1580Y] (ab224980)
- PE Anti-Cytokeratin 19 antibody [EP1580Y] (ab224981)
- Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/200.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) |
| Notes |
|---|
|
ICC/IF
1/200. This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) |
Target
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Function
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
Tissue specificity
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
Present in hair follicles at all stages of development. -
Domain
This keratin differs from all other IF proteins in lacking the C-terminal tail domain. - Information by UniProt
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Database links
- Entrez Gene: 3880 Human
- Entrez Gene: 16669 Mouse
- Omim: 148020 Human
- SwissProt: P08727 Human
- SwissProt: P19001 Mouse
- Unigene: 654568 Human
- Unigene: 439699 Mouse
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Alternative names
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203445)
ab203445 staining Cytokeratin 19 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203445 at 1/200 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).
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![Alexa Fluor® 568 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203445)](https://www.abcam.com/ps/products/203/ab203445/Images/ab203445-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 568 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203445)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab203445 has been referenced in 2 publications.
- Biasci D et al. CXCR4 inhibition in human pancreatic and colorectal cancers induces an integrated immune response. Proc Natl Acad Sci U S A 117:28960-28970 (2020). PubMed: 33127761
- Chen L et al. Combined use of EpCAM and FRa enables the high-efficiency capture of circulating tumor cells in non-small cell lung cancer. Sci Rep 8:1188 (2018). PubMed: 29352248