Recombinant Alexa Fluor® 568 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab203406)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 568 Rabbit monoclonal [EP4182] to Islet 1 - Neural Stem Cell Marker
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 568 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
See all Islet 1 primary antibodies -
Description
Alexa Fluor® 568 Rabbit monoclonal [EP4182] to Islet 1 - Neural Stem Cell Marker -
Host species
Rabbit -
Conjugation
Alexa Fluor® 568. Ex: 578nm, Em: 603nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: SKNSH cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP4182 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
- Alexa Fluor® 594 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab203404)
- Alexa Fluor® 555 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab203405)
- Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)
- Alexa Fluor® 488 Anti-Islet 1 antibody [EP4182] (ab275990)
- Alexa Fluor® 647 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab277925)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000.
This product gave a positive signal in SKNSH cells fixed with 4% formaldehyde (10 min) |
Notes |
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ICC/IF
1/1000. This product gave a positive signal in SKNSH cells fixed with 4% formaldehyde (10 min) |
Target
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Function
Binds to one of the cis-acting domain of the insulin gene enhancer. -
Tissue specificity
Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain. -
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3670 Human
- Entrez Gene: 16392 Mouse
- Entrez Gene: 64444 Rat
- Omim: 600366 Human
- SwissProt: P61371 Human
- SwissProt: P61372 Mouse
- SwissProt: P61374 Rat
- Unigene: 505 Human
see all -
Alternative names
- Insulin gene enhancer protein ISL 1 antibody
- Insulin gene enhancer protein ISL-1 antibody
- Insulin related protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab203406)
ab203406 staining Islet 1 in SKNSH cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203406 at a 1/1000 dilution (pseudocolored in green) and ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor® 647), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab203406 has not yet been referenced specifically in any publications.