Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Alexa Fluor® 568 Anti-mGluR1a antibody [EPR24160-173] (ab307772)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-mGluR1a antibody [EPR24160-173] (ab307772)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-mGluR1a antibody [EPR24160-173] (ab307772)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-mGluR1a antibody [EPR24160-173] (ab307772)
  • Alexa Fluor® 568 Anti-mGluR1a antibody [EPR24160-173] (ab307772)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 568 Rabbit monoclonal [EPR24160-173] to mGluR1a
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat
  • Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Carrier Free Unconjugated

Overview

  • Product name

    Alexa Fluor® 568 Anti-mGluR1a antibody [EPR24160-173]
    See all mGluR1a primary antibodies

  • Description

    Alexa Fluor® 568 Rabbit monoclonal [EPR24160-173] to mGluR1a

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 568. Ex: 578nm, Em: 603nm

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse and Rat cerebellum.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24160-173

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab307772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum.

  • Tissue specificity

    Detected in brain.

  • Involvement in disease

    Spinocerebellar ataxia, autosomal recessive, 13

  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Glutamate receptor metabotropic 1 antibody
      • GPRC1A antibody
      • GRM1 alpha antibody
      • GRM1 antibody
      • GRM1_HUMAN antibody
      • Metabotropic glutamate receptor 1 antibody
      • MGLU1 antibody
      • mGluR1 antibody
      • SCAR13 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-mGluR1a antibody [EPR24160-173] (ab307772)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-mGluR1a antibody [EPR24160-173] (ab307772)

      Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling mGluR1a with ab307772 at 1/50 dilution (10.0 µg/ml).


      Positive staining on mouse cerebellum.


      The section was incubated with ab307772 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-mGluR1a antibody [EPR24160-173] (ab307772)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-mGluR1a antibody [EPR24160-173] (ab307772)

      Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling mGluR1a with ab307772 at 1/50 dilution (10.0 µg/ml).


      Positive staining on rat cerebellum.


      The section was incubated with ab307772 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-mGluR1a antibody [EPR24160-173] (ab307772)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 568 Anti-mGluR1a antibody [EPR24160-173] (ab307772)

      Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling mGluR1a with ab307772 at 1/50 dilution (10.0 µg/ml).


      Negative control: no staining on mouse lung.


      The section was incubated with ab307772 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 568 Anti-mGluR1a antibody [EPR24160-173] (ab307772)
      Alexa Fluor® 568 Anti-mGluR1a antibody [EPR24160-173] (ab307772)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab307772? Please let us know so that we can cite the reference in this datasheet.

    ab307772 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab307772.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com