Recombinant Alexa Fluor® 568 Anti-Smad2 antibody [EP784Y] (ab312959)
![Alexa Fluor® 568 Anti-Smad2 antibody [EP784Y] (ab312959)](https://www.abcam.com/ps/products/312/ab312959/Images/ab312959-1-MTOConjugatedRecombinantAntibodyBenefits.jpg "Alexa Fluor® 568 Anti-Smad2 antibody [EP784Y] (ab312959)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 568 Rabbit monoclonal [EP784Y] to Smad2
- Suitable for: Target binding affinity, Antibody labelling
- Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 568 Anti-Smad2 antibody [EP784Y]
See all Smad2 primary antibodies -
Description
Alexa Fluor® 568 Rabbit monoclonal [EP784Y] to Smad2 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 568. Ex: 578nm, Em: 603nm -
Tested applications
Suitable for: Target binding affinity, Antibody labellingmore details -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for Abtrial: learn more here.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP784Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Smad2 antibody [EP784Y] - BSA and Azide free (ab157371)
- APC Anti-Smad2 antibody [EP784Y] (ab310863)
- Alexa Fluor® 647 Anti-Smad2 antibody [EP784Y] (ab311087)
- Alexa Fluor® 594 Anti-Smad2 antibody [EP784Y] (ab311684)
- Alexa Fluor® 555 Anti-Smad2 antibody [EP784Y] (ab313168)
- Anti-Smad2 antibody [EP784Y] (ab40855)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab312959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Target binding affinity |
Use at an assay dependent concentration.
|
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| Antibody labelling |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Target binding affinity
Use at an assay dependent concentration. |
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Antibody labelling
Use at an assay dependent concentration. |
Target
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Function
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. -
Tissue specificity
Expressed at high levels in skeletal muscle, heart and placenta. -
Sequence similarities
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
Post-translational
modificationsPhosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. - Information by UniProt
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Alternative names
- Drosophila, homolog of, MADR2 antibody
- hMAD-2 antibody
- HsMAD2 antibody
see all
Images
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Alexa Fluor® 568 Anti-Smad2 antibody [EP784Y] (ab312959)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab312959 has not yet been referenced specifically in any publications.