Validated using a knockout cell lineRecombinantRabMAb

Recombinant Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)

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Flow Cytometry (Intracellular) - Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)
  • Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 568 Rabbit monoclonal [EPR3776] to Vimentin - Cytoskeleton Marker
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Human
  • Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP PE Unconjugated

Overview

  • Product name

    Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker
    See all Vimentin primary antibodies

  • Description

    Alexa Fluor® 568 Rabbit monoclonal [EPR3776] to Vimentin - Cytoskeleton Marker

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 568. Ex: 578nm, Em: 603nm

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: NIH3T3, HeLa and wildtype HAP1 cells. Flow Cyt (intra): wildtype HAP1 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab202504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/1000.

This product gave a positive signal in NIH3T3 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) and HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)
Flow Cyt (Intra)
Use a concentration of 0.1 µg/ml.
Notes
ICC/IF
1/1000.

This product gave a positive signal in NIH3T3 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) and HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)
Flow Cyt (Intra)
Use a concentration of 0.1 µg/ml.

Target

  • Function

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

  • Tissue specificity

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

  • Involvement in disease

    Cataract 30

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Domain

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

  • Post-translational
    modifications

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Form

    Vimentin is found in connective tissue and in the cytoskeleton.

  • Alternative names

    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Images

  • Flow Cytometry (Intracellular) - Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)
    Flow Cytometry (Intracellular) - Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)

    Overlay histogram showing HAP1 wildtype (green line) and HAP1-VIM  knockout cells (red line) stained with ab202504. The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab202504, 0.1µg/ml dilution) for 30 min at 22°C.

    A rabbit monoclonal IgG isotype control antibody  (ab209613) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-VIM knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody can also be used in HAP1 cells fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)

    ab202504 staining Vimentin in wild-type HAP1 cells (top panel) and Vimentin knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202504 at a 1/1000 dilution (shown in green) and ab195884, Rat monoclonal [YOL1/34] to Tubulin (Alexa Fluor® 647), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)

    ab202504 staining Vimentin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202504 at 1/1000 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)

    ab202504 staining Vimentin in NIH3T3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202504 at a 1/1000 dilution (shown in pseudocolor green) and ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor® 647), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in NIH3T3 cells fixed with 100% methanol (5 min).

  • Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)
    Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab202504)

References (2)

Publishing research using ab202504? Please let us know so that we can cite the reference in this datasheet.

ab202504 has been referenced in 2 publications.

  • Palafox M  et al. High p16 expression and heterozygous RB1 loss are biomarkers for CDK4/6 inhibitor resistance in ER+ breast cancer. Nat Commun 13:5258 (2022). PubMed: 36071033
  • Li Y  et al. Triggering of Autophagy by Baicalein in Response to Apoptosis after Spinal Cord Injury: Possible Involvement of the PI3K Activation. Biol Pharm Bull 41:478-486 (2018). PubMed: 29367475

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (Alexa Fluor® 568)

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
Mammary gland
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Mar 26 2019

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