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Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (ab202368)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (ab202368)

    Key features and details

    • Alexa Fluor® 594 Mouse monoclonal [1A4] to alpha smooth muscle Actin
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
    • Isotype: IgG2a

    Conjugates logo Related conjugates and formulations

    Carrier Free

    Overview

    • Product name

      Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4]
      See all alpha smooth muscle Actin primary antibodies

    • Description

      Alexa Fluor® 594 Mouse monoclonal [1A4] to alpha smooth muscle Actin

    • Host species

      Mouse

    • Conjugation

      Alexa Fluor® 594. Ex: 590nm, Em: 617nm

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Sheep, Goat, Chicken, Guinea pig, Cat, Dog, Snake, Zebrafish

    • Positive control

      • ICC/IF: SV40LT-SMC cells.

    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab202368 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    1/200.

    This product gave a positive signal in SV40LT-SMC cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

    ab178001 - Mouse monoclonal IgG2a (Alexa Fluor® 594), is suitable for use as an isotype control with this antibody.
    Notes
    ICC/IF
    1/200.

    This product gave a positive signal in SV40LT-SMC cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

    ab178001 - Mouse monoclonal IgG2a (Alexa Fluor® 594), is suitable for use as an isotype control with this antibody.

    Target

    • Function

      Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

    • Involvement in disease

      Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.

    • Sequence similarities

      Belongs to the actin family.

    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • a actin antibody
      • AAT6 antibody
      • ACTA_HUMAN antibody
      • ACTA2 antibody
      • Actin alpha 2 smooth muscle aorta antibody
      • Actin aortic smooth muscle antibody
      • Actin, aortic smooth muscle antibody
      • ACTSA antibody
      • ACTVS antibody
      • Alpha 2 actin antibody
      • Alpha actin 2 antibody
      • Alpha cardiac actin antibody
      • alpha sma antibody
      • Alpha-actin-2 antibody
      • Cell growth inhibiting gene 46 protein antibody
      • Cell growth-inhibiting gene 46 protein antibody
      • GIG46 antibody
      • Growth inhibiting gene 46 antibody
      • MYMY5 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (ab202368)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (ab202368)

      ab202368 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202368 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This product also gave a positive signal under the same testing conditions in SV40LT-SMC cells fixed with 100% methanol (5min).

    References (8)

    Publishing research using ab202368? Please let us know so that we can cite the reference in this datasheet.

    ab202368 has been referenced in 8 publications.

    • Chung JJ  et al. Therapeutic Efficacy of Cryopreserved, Allogeneic Extracellular Vesicles for Treatment of Acute Myocardial Infarction. Int Heart J 62:381-389 (2021). PubMed: 33731514
    • Remes A  et al. Alginate hydrogel polymers enable efficient delivery of a vascular-targeted AAV vector into aortic tissue. Mol Ther Methods Clin Dev 21:83-93 (2021). PubMed: 33768132
    • Chen MH  et al. Injectable Supramolecular Hydrogel/Microgel Composites for Therapeutic Delivery. Macromol Biosci 19:e1800248 (2019). PubMed: 30259658
    • Rana BMJ  et al. A stromal cell niche sustains ILC2-mediated type-2 conditioning in adipose tissue. J Exp Med 216:1999-2009 (2019). PubMed: 31248899
    • Arunsan P  et al. Programmed knockout mutation of liver fluke granulin attenuates virulence of infection-induced hepatobiliary morbidity. Elife 8:N/A (2019). PubMed: 30644359
    • Ale-Agha N  et al. CDKN1B/p27 is localized in mitochondria and improves respiration-dependent processes in the cardiovascular system-New mode of action for caffeine. PLoS Biol 16:e2004408 (2018). PubMed: 29927970
    • Burwinkel M  et al. Intravenous injection of beta-amyloid seeds promotes cerebral amyloid angiopathy (CAA). Acta Neuropathol Commun 6:23 (2018). PubMed: 29506560
    • Capone C  et al. Mechanistic insights into a TIMP3-sensitive pathway constitutively engaged in the regulation of cerebral hemodynamics. Elife 5:N/A (2016). IHC ; Mouse . PubMed: 27476853

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