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RecombinantRabMAb

Recombinant Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] (ab201732)

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Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] (ab201732)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] (ab201732)
  • Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] (ab201732)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 594 Rabbit monoclonal [EPR1034Y] to GFAP
  • Suitable for: IHC-Fr
  • Reacts with: Mouse, Rat
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm

Conjugates logo Related conjugates and formulations

APC HRP PE Unconjugated

Overview

  • Product name

    Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y]
    See all GFAP primary antibodies

  • Description

    Alexa Fluor® 594 Rabbit monoclonal [EPR1034Y] to GFAP

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm

  • Tested applications

    Suitable for: IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-Fr: rat frozen dentate gyrus tissue section, mouse cerebellum

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab201732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
1/50 - 1/100.

This product gave a positive signal in rat frozen dentate gyrus tissue fixed with 10% formaldehyde (10 min).
Notes
IHC-Fr
1/50 - 1/100.

This product gave a positive signal in rat frozen dentate gyrus tissue fixed with 10% formaldehyde (10 min).

Target

  • Function

    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

  • Tissue specificity

    Expressed in cells lacking fibronectin.

  • Involvement in disease

    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Post-translational
    modifications

    Phosphorylated by PKN1.

  • Cellular localization

    Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] (ab201732)
    Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] (ab201732)

    IHC image of GFAP staining in a section of frozen normal rat dentate gyrus.

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab201732 at 1/100 (pseudocolored in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] (ab201732)
    Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] (ab201732)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling GFAP with ab201732 at 1/50 (10 ug/ml) dilution. The nuclear counterstain was DAPI (Blue).

  • Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] (ab201732)
    Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] (ab201732)

References (0)

Publishing research using ab201732? Please let us know so that we can cite the reference in this datasheet.

ab201732 has not yet been referenced specifically in any publications.

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