Recombinant Alexa Fluor® 594 Anti-Huntingtin antibody [EPR5526] (ab302693)
![Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-Huntingtin antibody [EPR5526] (AB302693)](https://www.abcam.com/ps/products/302/ab302693/Images/AB302693-490070-anti-huntingtin-antibody-epr5526-ihc-frozen-mouse-cerebellum.jpg "Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-Huntingtin antibody [EPR5526] (AB302693)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 594 Rabbit monoclonal [EPR5526] to Huntingtin
- Suitable for: IHC-Fr
- Reacts with: Mouse, Rat
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 594 Anti-Huntingtin antibody [EPR5526]
See all Huntingtin primary antibodies -
Description
Alexa Fluor® 594 Rabbit monoclonal [EPR5526] to Huntingtin -
Host species
Rabbit -
Conjugation
Alexa Fluor® 594. Ex: 590nm, Em: 617nm -
Tested applications
Suitable for: IHC-Frmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat
Does not react with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab271195) -
Positive control
- IHC-Fr: Mouse and rat cerebellum frozen (fresh) tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5526 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunizing Peptide (Blocking)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab302693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/50.
|
| Notes |
|---|
|
IHC-Fr
1/50. |
Target
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Function
May play a role in microtubule-mediated transport or vesicle function. -
Tissue specificity
Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. -
Involvement in disease
Defects in HTT are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum). -
Sequence similarities
Belongs to the huntingtin family.
Contains 10 HEAT repeats. -
Domain
The N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations. -
Post-translational
modificationsCleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis.
Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington's disease neurons. - Information by UniProt
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Database links
- Entrez Gene: 15194 Mouse
- Entrez Gene: 29424 Rat
- SwissProt: P42859 Mouse
- SwissProt: P51111 Rat
- Unigene: 209071 Mouse
- Unigene: 482929 Mouse
- Unigene: 11193 Rat
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Alternative names
- AI256365 antibody
- C430023I11Rik antibody
- HD antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-Huntingtin antibody [EPR5526] (AB302693)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum (fresh) tissue labeling Huntingtin with ab302693 at 1/50 (10.0 μg/ml) dilution (Red). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue). Negative control: Primary diluent was used instead of primary antibody.
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![Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-Huntingtin antibody [EPR5526] (AB302693)](https://www.abcam.com/ps/products/302/ab302693/Images/AB302693-490071-anti-huntingtin-antibody-epr5526-ihc-frozen-rat-cerebellum.jpg)
Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-Huntingtin antibody [EPR5526] (AB302693)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum (fresh) tissue labeling Huntingtin with ab302693 at 1/50 (10.0 μg/ml) dilution (Red). Positive staining on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue). Negative control: Primary diluent was used instead of primary antibody.
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![Alexa Fluor® 594 Anti-Huntingtin antibody [EPR5526] (AB302693)](https://www.abcam.com/ps/products/302/ab302693/Images/AB302693-1-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 594 Anti-Huntingtin antibody [EPR5526] (AB302693)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab302693 has not yet been referenced specifically in any publications.