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    products/primary-antibodies/alexa-fluor-594-mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab178002.pdf

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Alexa Fluor® 594 Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab178002)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab178002)

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    Overview

    • Product name

      Alexa Fluor® 594 Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control
    • Conjugation

      Alexa Fluor® 594. Ex: 590nm, Em: 617nm
    • Specificity

      ab178002 was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments. ab178002 is a suitable control for Human, Rat, Mouse, Monkey, others untested.
    • Tested applications

      Suitable for: ICC/IFmore details
    • General notes

      Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The Control performed appropriately in all materials and platforms that were tested.

      Product was previously marketed under the MitoSciences sub-brand.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Store In the Dark.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
    • Concentration information loading...
    • Purity

      Ammonium Sulphate Precipitation
    • Clonality

      Monoclonal
    • Clone number

      7E10G10
    • Isotype

      IgG2b
    • Light chain type

      kappa

    Associated products

    • Alternative Versions

      • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
      • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free (ab221850)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab178002 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    Use at an assay dependent concentration.
    Notes
    ICC/IF
    Use at an assay dependent concentration.

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab178002)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab178002)

      Immunocytochemistry data for ab178002. All panels show HeLa cells stained with DAPI as well as; a no primary control (A), ab178002 at 1 μg/mL (B), an Alexa 594 anti-SDHA conjugated antibody (ab170172) at 1 μg/mL (C).

      Cells were PFA fixed and stored in 0.02%azide/PBS in 4°C. Cells were blocked with 10%GS for 2 hours, primary was diluted in 10%GS and incubated for 2 hours, cells were washed 3X with 1%GS and DAPI was added for 10 minutes in 1%GS.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab178002? Please let us know so that we can cite the reference in this datasheet.

    ab178002 has not yet been referenced specifically in any publications.

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