Recombinant Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)](https://www.abcam.com/ps/products/305/ab305372/Images/ab305372-3-anti-myelin-oligodendrocyte-glycoprotein-antibody-epr22629-310-immunohistochemistry-human-cerebellum.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 594 Rabbit monoclonal [EPR22629-310] to Myelin oligodendrocyte glycoprotein
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Mouse, Rat, Human
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310]
See all Myelin oligodendrocyte glycoprotein primary antibodies -
Description
Alexa Fluor® 594 Rabbit monoclonal [EPR22629-310] to Myelin oligodendrocyte glycoprotein -
Host species
Rabbit -
Conjugation
Alexa Fluor® 594. Ex: 590nm, Em: 617nm -
Tested applications
Suitable for: IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human, mouse and rat cerebellum tissue. IHC-Fr: Mouse and rat cerebellum tissue (fresh).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22629-310 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab305372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/50.
|
|
| IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
IHC-Fr
1/50. |
|
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication. -
Tissue specificity
Found exclusively in the CNS, where it is localized on the surface of myelin and oligodendrocyte cytoplasmic membranes. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4340 Human
- Entrez Gene: 17441 Mouse
- Entrez Gene: 24558 Rat
- Omim: 159465 Human
- SwissProt: Q16653 Human
- SwissProt: Q61885 Mouse
- SwissProt: Q63345 Rat
- Unigene: 141308 Human
see all -
Alternative names
- BTN6 antibody
- BTNL11 antibody
- MGC26137 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab305372 at 1/50 dilution (10.0 ug/ml).
Positive staining on human cerebellum. The section was incubated with ab305372 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)](https://www.abcam.com/ps/products/305/ab305372/Images/ab305372-4-anti-myelin-oligodendrocyte-glycoprotein-antibody-epr22629-310-immunohistochemistry-mouse-cerebellum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab305372 at 1/50 dilution (10.0 ug/ml).
Positive staining on mouse cerebellum. The section was incubated with ab305372 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)](https://www.abcam.com/ps/products/305/ab305372/Images/ab305372-5-anti-myelin-oligodendrocyte-glycoprotein-antibody-epr22629-310-immunohistochemistry-rat-cerebellum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab305372 at 1/50 dilution (10.0 ug/ml).
Positive staining on rat cerebellum. The section was incubated with ab305372 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.
-
![Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)](https://www.abcam.com/ps/products/305/ab305372/Images/ab305372-1-anti-myelin-oligodendrocyte-glycoprotein-antibody-epr22629-310-immunohistochemistry-frozen-mouse-cerebellum-fresh.jpg)
Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum (fresh) tissue labeling Myelin oligodendrocyte glycoprotein with ab305372 at 1/500 dilution (Red).
Confocal image showing positive staining on mouse cerebellum.
The section was incubated with ab305372 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
-
![Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)](https://www.abcam.com/ps/products/305/ab305372/Images/ab305372-2-anti-myelin-oligodendrocyte-glycoprotein-antibody-epr22629-310-immunohistochemistry-frozen-rat-cerebellum-fresh.jpg)
Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab305372)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum (fresh) tissue labeling Myelin oligodendrocyte glycoprotein with ab305372 at 1/500 dilution (Red).
Confocal image showing positive staining on rat cerebellum.
The section was incubated with ab305372 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab305372 has not yet been referenced specifically in any publications.