Recombinant Alexa Fluor® 594 Anti-Olig2 antibody [EPR2673] (ab300729)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Olig2 antibody [EPR2673] (ab300729)](https://www.abcam.com/ps/products/300/ab300729/Images/AB300729-1-anti-olig2-antibody-epr2673-immunocytochemistry-rat-primary-neuroglia-cell.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Olig2 antibody [EPR2673] (ab300729)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 594 Rabbit monoclonal [EPR2673] to Olig2
- Suitable for: ICC/IF
- Reacts with: Rat
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 594 Anti-Olig2 antibody [EPR2673]
See all Olig2 primary antibodies -
Description
Alexa Fluor® 594 Rabbit monoclonal [EPR2673] to Olig2 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 594. Ex: 590nm, Em: 617nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: rat primary neural/glia cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2673 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/50.
|
| Notes |
|---|
|
ICC/IF
1/50. |
Target
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Function
Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. -
Tissue specificity
Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable. -
Involvement in disease
Note=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Domain
The bHLH is essential for interaction with NKX2-2. -
Cellular localization
Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2. - Information by UniProt
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Database links
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Alternative names
- Basic domain helix loop helix protein class B 1 antibody
- Basic helix loop helix protein class B 1 antibody
- BHLHB antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Olig2 antibody [EPR2673] (ab300729)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cells labelling Olig2 with ab300729 at 1/50 dilution (10.0 µg/mL) (Red). Confocal image showing positive staining in rat primary neural/glia cells. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 ug/ml dilution (Green). The Nuclear counterstain was DAPI (Blue).
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![Alexa Fluor® 594 Anti-Olig2 antibody [EPR2673] (AB300729)](https://www.abcam.com/ps/products/300/ab300729/Images/AB300729-2-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 594 Anti-Olig2 antibody [EPR2673] (AB300729)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab300729 has not yet been referenced specifically in any publications.