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RecombinantRabMAb

Recombinant Alexa Fluor® 594 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab206872)

Reviews (1) Submit a question References (1)
Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab206872)
  • Alexa Fluor® 594 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab206872)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 594 Rabbit monoclonal [Y92] to PDGFR alpha + PDGFR beta
  • Suitable for: Flow Cyt (Intra)
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Carrier Free HRP PE Unconjugated

Overview

  • Product name

    Alexa Fluor® 594 Anti-PDGFR alpha + PDGFR beta antibody [Y92]

  • Description

    Alexa Fluor® 594 Rabbit monoclonal [Y92] to PDGFR alpha + PDGFR beta

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm

  • Tested applications

    Suitable for: Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt (intra): NIH3T3 cells. WB: N-GST tagged Human PDGF Receptor beta (aa557 to 1106) recombinant protein, N-GST tagged Human PDGF Receptor alpha (aa550 to 1089) recombinant protein.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab206872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.
Notes
Flow Cyt (Intra)
1/500.

Target

Images

  • Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab206872)
    Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab206872)

    Overlay histogram showing NIH3T3 cells stained with ab206872 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab206872, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 594 (ab208568) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 610/20 bandpass filter.

    This antibody gave a positive signal in NIH3T3 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Alexa Fluor® 594 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab206872)
    Alexa Fluor® 594 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab206872)

References (1)

Publishing research using ab206872? Please let us know so that we can cite the reference in this datasheet.

ab206872 has been referenced in 1 publication.

  • Bohannon DG  et al. A subtype of cerebrovascular pericytes is associated with blood-brain barrier disruption that develops during normal aging and simian immunodeficiency virus infection. Neurobiol Aging 96:128-136 (2020). PubMed: 33002766

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PDGF Receptor beta antibody [Y92] (Alexa Fluor® 594)

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Commercial antigen retrieval
Permeabilization
Yes - Triton 0.25%
Specification
Lung
Blocking step
(agent) for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 22 2016

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