Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)

Submit a review Submit a question
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)

Key features and details

  • Alexa Fluor® 594 Mouse monoclonal [2E3GC12FB2AE2] to SDHA
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 HRP Unconjugated

Overview

  • Product name

    Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2]
    See all SDHA primary antibodies

  • Description

    Alexa Fluor® 594 Mouse monoclonal [2E3GC12FB2AE2] to SDHA

  • Host species

    Mouse

  • Conjugation

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog

  • Immunogen

    Tissue, cells or virus corresponding to Cow SDHA. Purified mitochondrial complex II (Cow).
    Database link: P31039

  • Positive control

    • ICC/IF: HeLa and HDFn cells.

  • General notes

    ab170172 was previously used as a component in the MitoBiogenesis™ ICC Kit. The protocol for this kit is available here.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/1000.

ab178000 - Mouse monoclonal IgG1 (Alexa Fluor® 594), is suitable for use as an isotype control with this antibody.
Notes
ICC/IF
1/1000.

ab178000 - Mouse monoclonal IgG1 (Alexa Fluor® 594), is suitable for use as an isotype control with this antibody.

Target

  • Function

    Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).

  • Pathway

    Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.

  • Involvement in disease

    Defects in SDHA are a cause of mitochondrial complex II deficiency (MT-C2D) [MIM:252011]. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations. Clinical features include psychomotor regression in infants, poor growth with lack of speech development, severe spastic quadriplegia, dystonia, progressive leukoencephalopathy, muscle weakness, exercise intolerance, cardiomyopathy. Some patients manifest Leigh syndrome or Kearns-Sayre syndrome.
    Defects in SDHA are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
    Defects in SDHA are the cause of cardiomyopathy dilated type 1GG (CMD1GG) [MIM:613642]. CMD1GG is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

  • Sequence similarities

    Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CMD1GG antibody
    • DHSA_HUMAN antibody
    • Flavoprotein subunit of complex II antibody
    • Fp antibody
    • PGL5 antibody
    • SDH 2 antibody
    • SDH1 antibody
    • SDH2 antibody
    • SDHA antibody
    • SDHF antibody
    • Succinate dehydrogenase [ubiquinone] flavoprotein subunit antibody
    • Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial antibody
    • Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial antibody
    • Succinate dehydrogenase complex flavoprotein subunit A antibody
    • Succinate dehydrogenase complex flavoprotein subunit antibody
    • Succinate dehydrogenase complex flavoprotein subunit precursor antibody
    • Succinate dehydrogenase complex subunit A antibody
    • Succinate dehydrogenase complex subunit A flavoprotein (Fp) antibody
    • Succinate dehydrogenase complex subunit A flavoprotein antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)

    ab170172 staining SDHA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab170172 at a 1/1000 dilution (shown in pseudo color red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)

    Immunocytochemistry with HDFn (100x) cells.
    A) HDFn stained with anti-SDHA Alexa-594 antibody (1.0 µg/mL). B) HDFn stained with Anti-mitochondrial chaperone HSP60 (1/1000, ab46798), Secondary antibody used was goat anti-rabbit Alexa® 488 (1/1000, ab150077). C) DAPI as nuclear stain (1/10000). D) Merge of color channels to show specificity of signal to mitochondria.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)

    Immunocytochemistry with HeLa cells (100x).
    A) HeLa stained with anti-SDHA Alexa-594 antibody (1.0 µg/mL). B) HeLa stained with Anti-HSP60 (1/1000, ab46798), Secondary antibody used was goat anti-rabbit Alexa® 488 (1/1000, ab150077). C) DAPI as nuclear stain (1/10000). D) Merge of color channels to show specificity of signal to mitochondria.

References (0)

Publishing research using ab170172? Please let us know so that we can cite the reference in this datasheet.

ab170172 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab170172.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com