Recombinant Alexa Fluor® 647 Anti-ABCD1/ALD antibody [EPR15929] (ab225409)
![Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-ABCD1/ALD antibody [EPR15929] (ab225409)](https://www.abcam.com/ps/products/225/ab225409/Images/ab225409-341204-ab225409FLOW1.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-ABCD1/ALD antibody [EPR15929] (ab225409)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR15929] to ABCD1/ALD
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 647 Anti-ABCD1/ALD antibody [EPR15929]
See all ABCD1/ALD primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EPR15929] to ABCD1/ALD -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment within Human ABCD1/ALD aa 650 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P33897 -
Positive control
- ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15929 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
|
| ICC/IF |
1/100.
This product gave a positive signal in HepG2 cells fixed with 100% methanol (5 min) |
| Notes |
|---|
|
Flow Cyt (Intra)
1/500. |
|
ICC/IF
1/100. This product gave a positive signal in HepG2 cells fixed with 100% methanol (5 min) |
Target
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Function
Probable transporter. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity. -
Involvement in disease
Defects in ABCD1 are the cause of adrenoleukodystrophy X-linked (X-ALD) [MIM:300100]. X-ALD is a peroxisomal metabolic disorder characterized by progressive multifocal demyelination of the central nervous system and by peripheral adrenal insufficiency (Addison disease). It results in mental deterioration, corticospinal tract dysfunction, and cortical blindness. Different clinical manifestations exist like: cerebral childhood ALD (CALD), adult cerebral ALD (ACALD), adrenomyeloneuropathy (AMN) and 'Addison disease only' (ADO) phenotype.
Note=The promoter region of ABCD1 is deleted in the chromosome Xq28 deletion syndrome which involves ABCD1 and the neighboring gene BCAP31. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 1 ABC transporter domain. -
Cellular localization
Peroxisome membrane. - Information by UniProt
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Database links
- Entrez Gene: 215 Human
- Entrez Gene: 11666 Mouse
- Entrez Gene: 363516 Rat
- Omim: 300371 Human
- SwissProt: P33897 Human
- SwissProt: P48410 Mouse
- Unigene: 159546 Human
- Unigene: 365 Mouse
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Alternative names
- ABC42 antibody
- Abcd1 antibody
- ABCD1_HUMAN antibody
see all
Images
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Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-ABCD1/ALD antibody [EPR15929] (ab225409)
Overlay histogram showing HepG2 cells stained with ab225409 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225409, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ABCD1/ALD antibody [EPR15929] (ab225409)](https://www.abcam.com/ps/products/225/ab225409/Images/ab225409-319146-ICCAP00405621.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ABCD1/ALD antibody [EPR15929] (ab225409)ab225409 staining ABCD1/ALD in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225409 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Alexa Fluor® 647 Anti-ABCD1/ALD antibody [EPR15929] (ab225409)](https://www.abcam.com/ps/products/225/ab225409/Images/ab225409-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-ABCD1/ALD antibody [EPR15929] (ab225409)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab225409 has not yet been referenced specifically in any publications.