Recombinant Alexa Fluor® 647 Anti-ATG3 antibody [EPR4801] (ab310185)
![Alexa Fluor® 647 Anti-ATG3 antibody [EPR4801] (ab310185)](https://www.abcam.com/ps/products/310/ab310185/Images/ab310185-1-MTOConjugatedRecombinantAntibodyBenefits.jpg "Alexa Fluor® 647 Anti-ATG3 antibody [EPR4801] (ab310185)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR4801] to ATG3
- Suitable for: Antibody labelling, Target binding affinity
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 647 Anti-ATG3 antibody [EPR4801]
See all ATG3 primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EPR4801] to ATG3 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: Antibody labelling, Target binding affinitymore details -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for Abtrial: learn more here.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4801 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-ATG3 antibody [EPR4801] - BSA and Azide free (ab226019)
- PE Anti-ATG3 antibody [EPR4801] (ab306349)
- APC Anti-ATG3 antibody [EPR4801] (ab306350)
- HRP Anti-ATG3 antibody [EPR4801] (ab306351)
- AP Anti-ATG3 antibody [EPR4801] (ab308899)
- Alexa Fluor® 555 Anti-ATG3 antibody [EPR4801] (ab312153)
- Alexa Fluor® 568 Anti-ATG3 antibody [EPR4801] (ab312639)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab310185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Antibody labelling |
Use at an assay dependent concentration.
|
|
| Target binding affinity |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Antibody labelling
Use at an assay dependent concentration. |
|
Target binding affinity
Use at an assay dependent concentration. |
Target
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Function
E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. -
Tissue specificity
Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. -
Sequence similarities
Belongs to the ATG3 family. -
Post-translational
modificationsConjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- 2610016C12Rik antibody
- Apg 3 antibody
- APG3 antibody
see all
Images
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Alexa Fluor® 647 Anti-ATG3 antibody [EPR4801] (ab310185)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab310185 has not yet been referenced specifically in any publications.