Recombinant Alexa Fluor® 647 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab310016)
![Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab310016)](https://www.abcam.com/ps/products/310/ab310016/Images/ab310016-521702-alexa-fluor-647-anti-cd3-zeta-phospho-y142-antibody-ep265-2-y-flow-cytometry-jurkat-cells-human.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab310016)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EP265(2)Y] to CD3 zeta (phospho Y142)
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 647 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y]
See all CD3 zeta primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EP265(2)Y] to CD3 zeta (phospho Y142) -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Jurkat cells treated with pervanadate (1 mM) for 30 min Flow Cyto (intra): Jurkat (human T cell leukemia T lymphocyte from peripheral blood) treated with 1mM Pervanadate for 30min
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP265(2)Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)
- PE Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab305800)
- APC Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab305801)
- Alexa Fluor® 555 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab311910)
- Alexa Fluor® 568 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab312381)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab310016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Function
Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. -
Involvement in disease
Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens. -
Sequence similarities
Belongs to the CD3Z/FCER1G family.
Contains 3 ITAM domains. -
Domain
The ITAM domains mediate interaction with SHB. -
Post-translational
modificationsPhosphorylated on Tyr residues after T-cell receptor triggering. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 919 Human
- Omim: 186780 Human
- SwissProt: P20963 Human
- Unigene: 156445 Human
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Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab310016)
Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood) treated with 1mM Pervanadate for 30min (Red) / Untreated Jurkat (Green) cells labelling CD3 zeta (phospho Y142) with ab310016 at 1/500 dilution (0.1 ug) /(Red and Green) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab310016)](https://www.abcam.com/ps/products/310/ab310016/Images/ab310016-521701-alexa-fluor-647-anti-cd3-zeta-phospho-y142-antibody-ep265-2-y-immunocytochemistry-jurkat-cells-human.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab310016)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood) cells labelling CD3 zeta (phospho Y142) with ab310016 at 1/50 dilution (10.0 µg/ml). ab195887, Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used at 1/200 dilution (2.5 µg/ml) for Tubulin counter stanining (Green). DAPI was used as nuclear counter stain.
Confocal image showing increased cytoplasmic staining in Jurkat cells treated with pervanadate (1 mM) for 30 min.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). -
![Alexa Fluor® 647 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab310016)](https://www.abcam.com/ps/products/310/ab310016/Images/ab310016-1-MTOConjugatedRecombinantAntibodyBenefits.jpg)
Alexa Fluor® 647 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (ab310016)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab310016 has not yet been referenced specifically in any publications.