Recombinant Alexa Fluor® 647 Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y-129R] (ab199819)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CDKN2A/p16INK4A +CDKN2B/p15INK4B antibody [EP435Y-129R] (ab199819)](https://www.abcam.com/ps/products/199/ab199819/Images/ab199819-482815-ICC-199819.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CDKN2A/p16INK4A +CDKN2B/p15INK4B antibody [EP435Y-129R] (ab199819)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EP435Y-129R] to CDKN2A/p16INK4A +CDKN2B/p15INK4B
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 647 Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y-129R]
See all CDKN2A/p16INK4A +CDKN2B/p15INK4B primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EP435Y-129R] to CDKN2A/p16INK4A +CDKN2B/p15INK4B -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human CDKN2A/p16INK4a+CDKN2A/p16INK4A +CDKN2B/p15INK4B.
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Positive control
- ICC/IF: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 68.98% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP435Y-129R -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/200.
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| Notes |
|---|
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ICC/IF
1/200. |
Target
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Function
Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. -
Tissue specificity
Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific. -
Involvement in disease
The association between cutaneous and uveal melanomas in some families suggests that mutations in CDKN2A may account for a proportion of uveal melanomas. However, CDKN2A mutations are rarely found in uveal melanoma patients.
Melanoma, cutaneous malignant 2
Familial atypical multiple mole melanoma-pancreatic carcinoma syndrome
Melanoma-astrocytoma syndrome -
Sequence similarities
Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
Contains 4 ANK repeats. -
Post-translational
modificationsPhosphorylation seems to increase interaction with CDK4. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1029 Human
- SwissProt: P42771 Human
- Unigene: 512599 Human
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Alternative names
- CDK4I antibody
- CDKN2A antibody
- CDN2A_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CDKN2A/p16INK4A +CDKN2B/p15INK4B antibody [EP435Y-129R] (ab199819)
ab199819 staining CDKN2A/p16INK4a in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199819 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Alexa Fluor® 647 Anti-CDKN2A/p16INK4A +CDKN2B/p15INK4B antibody [EP435Y-129R] (ab199819)](https://www.abcam.com/ps/products/199/ab199819/Images/ab199819-417385-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 647 Anti-CDKN2A/p16INK4A +CDKN2B/p15INK4B antibody [EP435Y-129R] (ab199819)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab199819 has not yet been referenced specifically in any publications.