Alexa Fluor® 647 Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab197491)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab197491)](https://www.abcam.com/ps/products/197/ab197491/Images/ab197491-239503-anti-cox-iv-antibody-mabcam33985-mitochondrial-marker-alexa-fluor-647-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab197491)")
Key features and details
- Alexa Fluor® 647 Mouse monoclonal [mAbcam33985] to COX IV - Mitochondrial Marker
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG1
Related conjugates and formulations
Overview
-
Product name
Alexa Fluor® 647 Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker
See all COX IV primary antibodies -
Description
Alexa Fluor® 647 Mouse monoclonal [mAbcam33985] to COX IV - Mitochondrial Marker -
Host species
Mouse -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: HeLa cells.
-
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
mAbcam33985 -
Myeloma
Sp2 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/1000.
|
| Notes |
|---|
|
ICC/IF
1/1000. |
Target
-
Function
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the cytochrome c oxidase IV family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
-
Database links
- Entrez Gene: 1327 Human
- Omim: 123864 Human
- SwissProt: P13073 Human
- Unigene: 433419 Human
-
Alternative names
- AL024441 antibody
- COX 4 antibody
- COX IV 1 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab197491)
ab197491 staining COX IV in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197491 at 1/1000 dilution (shown in red) and ab6046 (Rabbit polyclonal to beta Tubulin) at 1µg/ml. This was followed by an incubation at room temperature for 1h with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed, at 1µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab197491 has not yet been referenced specifically in any publications.