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    products/primary-antibodies/alexa-fluor-647-cox1--cyclooxygenase-1-antibody-epr5866-ab199202.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199202)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199202)
  • Alexa Fluor® 647 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199202)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR5866] to COX1 / Cyclooxygenase 1
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free HRP PE Unconjugated

You may also be interested in

Protein
Product image
Recombinant human COX1 / Cyclooxygenase 1 protein (ab198643)
Primary
Product image
Alexa Fluor® 488 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199027)
Conjugation
Product image
Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (ab269823)

View more associated products

Overview

  • Product name

    Alexa Fluor® 647 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866]
    See all COX1 / Cyclooxygenase 1 primary antibodies
  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR5866] to COX1 / Cyclooxygenase 1
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • NIH3T3, HACAT, Neuro -2a, C2C12, A431, and L6 cell lysates; Human skin tissue; HeLa cells. ICC/IF: HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Dissociation constant (KD)

    KD = 5.50 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5866
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Platelets
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Prostaglandins
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Heart disease
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    • Alexa Fluor® 488 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199027)
    • HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203)
    • PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab209581)
    • Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] - BSA and Azide free (ab219375)
  • Isotype control

    • Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093)
  • Recombinant Protein

    • Recombinant human COX1 / Cyclooxygenase 1 protein (ab198643)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab199202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/1000.

This product gave a positive signal in 100% methanol (5 min)

Notes
ICC/IF
1/1000.

This product gave a positive signal in 100% methanol (5 min)

Target

  • Function

    May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
  • Pathway

    Lipid metabolism; prostaglandin biosynthesis.
  • Sequence similarities

    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Cellular localization

    Microsome membrane. Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession P23219 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5742 Human
    • Entrez Gene: 19224 Mouse
    • Entrez Gene: 24693 Rat
    • Omim: 176805 Human
    • SwissProt: P23219 Human
    • SwissProt: P22437 Mouse
    • SwissProt: Q63921 Rat
    • Unigene: 201978 Human
    • Unigene: 275434 Mouse
    • Unigene: 44404 Rat
    see all
  • Alternative names

    • COX 1 antibody
    • COX 3 antibody
    • COX-1 antibody
    • COX1 antibody
    • Cox3 antibody
    • Cyclooxygenase 1 antibody
    • Cyclooxygenase 3, included antibody
    • Cyclooxygenase-1 antibody
    • EC 1.14.99.1 antibody
    • Partial COX1 proteins, included antibody
    • PCOX1 antibody
    • PGG/HS antibody
    • PGH synthase 1 antibody
    • PGH1_HUMAN antibody
    • PGHS 1 antibody
    • PGHS-1 antibody
    • PGHS1 antibody
    • PHS 1 antibody
    • PHS1 antibody
    • Prostaglandin endoperoxide synthase 1 antibody
    • Prostaglandin G/H synthase 1 antibody
    • Prostaglandin H2 synthase 1 antibody
    • Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 1 antibody
    • PTGHS antibody
    • PTGS1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199202)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199202)

    ab199202 staining COX1 / Cyclooxygenase 1 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199202 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 647 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199202)
    Alexa Fluor® 647 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199202)

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab199202? Please let us know so that we can cite the reference in this datasheet.

ab199202 has not yet been referenced specifically in any publications.

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