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    products/primary-antibodies/alexa-fluor-647-cytochrome-c-antibody-37ba11-ab198583.pdf

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Alexa Fluor® 647 Anti-Cytochrome C antibody [37BA11] (ab198583)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytochrome C antibody [37BA11] (ab198583)

    Key features and details

    • Alexa Fluor® 647 Mouse monoclonal [37BA11] to Cytochrome C
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG2a

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 488 HRP Unconjugated

    You may also be interested in

    Primary
    Product image
    Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
    Protein
    Recombinant Human Cytochrome C protein (ab172817)
    Primary
    Product image
    Alexa Fluor® 647 Anti-Cytochrome C antibody [EPR1327] (ab192486)

    View more associated products

    Overview

    • Product name

      Alexa Fluor® 647 Anti-Cytochrome C antibody [37BA11]
      See all Cytochrome C primary antibodies
    • Description

      Alexa Fluor® 647 Mouse monoclonal [37BA11] to Cytochrome C
    • Host species

      Mouse
    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Cow, Caenorhabditis elegans
    • Immunogen

      Full length native protein (purified) corresponding to Cow Cytochrome C. Bovine heart Cytochrome C.

    • Positive control

      • ICC/IF: HeLa cells.
    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
    • Concentration information loading...
    • Purity

      Affinity purified
    • Purification notes

      ab110325 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
    • Clonality

      Monoclonal
    • Clone number

      37BA11
    • Isotype

      IgG2a
    • Light chain type

      kappa
    • Research areas

      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipid Metabolism
      • Cytochromes
      • Cell Biology
      • Apoptosis
      • Mitochondrial
      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Signal Transduction
      • Metabolism
      • Mitochondrial
      • Cancer
      • Invasion/microenvironment
      • Apoptosis
      • Cytochrome C
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Integration of energy metabolism
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Oxidative phosphorylation
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial markers
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipases
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Integration of energy
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Cytochromes
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Apoptosis
      • Cancer
      • Cell Death
      • Apoptosis
      • Apoptosis Markers
      • Cytochrome C
      • Cancer
      • Cell Death
      • Apoptosis
      • Mitochondrial
      • Cancer
      • Cell Death
      • Apoptosis
      • Metabolism

    Associated products

    • Alternative Versions

      • Anti-Cytochrome C antibody [37BA11] (ab110325)
      • Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
      • HRP Anti-Cytochrome C antibody [37BA11] (ab198489)
    • Recombinant Protein

      • Recombinant Human Cytochrome C protein (ab172817)
    • Related Products

      • Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab198583 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    1/100.

    This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

    Notes
    ICC/IF
    1/100.

    This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

    Target

    • Function

      Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
      Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
    • Involvement in disease

      Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
    • Sequence similarities

      Belongs to the cytochrome c family.
    • Post-translational
      modifications

      Binds 1 heme group per subunit.
    • Cellular localization

      Mitochondrion matrix.
    • Target information above from: UniProt accession P99999 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 510767 Cow
      • Entrez Gene: 54205 Human
      • Entrez Gene: 13063 Mouse
      • Entrez Gene: 25309 Rat
      • Omim: 123970 Human
      • SwissProt: P62894 Cow
      • SwissProt: P99999 Human
      • SwissProt: P62897 Mouse
      • SwissProt: P62898 Rat
      • Unigene: 437060 Human
      • Unigene: 617193 Human
      see all
    • Alternative names

      • CYC antibody
      • CYC_HUMAN antibody
      • CYCS antibody
      • Cytochrome c antibody
      • Cytochrome c somatic antibody
      • HCS antibody
      • THC4 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytochrome C antibody [37BA11] (ab198583)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytochrome C antibody [37BA11] (ab198583)

      ab198583 staining Cytochrome C in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198583 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab198583? Please let us know so that we can cite the reference in this datasheet.

    ab198583 has been referenced in 2 publications.

    • Zhou H  et al. The oncolytic compound LTX-401 targets the Golgi apparatus. Cell Death Differ 23:2031-2041 (2016). PubMed: 27588704
    • Zhou H  et al. The oncolytic peptide LTX-315 kills cancer cells through Bax/Bak-regulated mitochondrial membrane permeabilization. Oncotarget 6:26599-614 (2015). PubMed: 26378049

    Customer reviews and Q&As

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