Recombinant Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245893)
![Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245893)](https://www.abcam.com/ps/products/245/ab245893/Images/ab245893-391589-AntiDDDDK-FLAG-tag-antibody-EPR20018251-Alexa-Fluor-647-flow-cytometry-transfected-293t.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245893)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR20018-251] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: Flow Cyt (Intra), ICC/IF
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EPR20018-251] to DDDDK tag (Binds to FLAG® tag sequence) -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IFmore details -
Species reactivity
Predicted to work with: Species independent
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: FLAG-tagged PFKFB3 expression construct transfected 293T cells. Flow Cyt (intra): 293T transfected with DDDDK-tagged PDL1 expression construct cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20018-251 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
|
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| ICC/IF |
1/50.
This product gave a positive signal in FLAG®-tagged PFKFB3 expression construct transfected 293T cells fixed with 4% formaldehyde (10 min). |
| Notes |
|---|
|
Flow Cyt (Intra)
1/50. |
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ICC/IF
1/50. This product gave a positive signal in FLAG®-tagged PFKFB3 expression construct transfected 293T cells fixed with 4% formaldehyde (10 min). |
Target
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Relevance
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
Alternative names
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
Images
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Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245893)
Intracellular flow cytometric analysis of 4% PFA-fixed, 90% methanol-permeabilized 293T transfected with DDDDK-tagged PDL1 expression construct cells labeling DDDDK tag with ab245893 at 1/50 dilution (red) compared with ab199093 Rabbit IgG, monoclonal [EPR25A] - Isotype Control Alexa Fluor® 647 (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245893)](https://www.abcam.com/ps/products/245/ab245893/Images/ab245893-350887-anti-ddddk-tag-epr20018251-alexa-647-immunocytochemistry-293T-cells-transfected-with-a-FLAGtagged-PFKFB3-expression-vector.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245893)ab245893 staining DDDDK tag in 293T cells transfected with a FLAG®-tagged PFKFB3 expression vector. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab245893 at 1/50 dilution (shown in red) and a Mouse monoclonal anti-FLAG® M2 antibody used as a counterstain. This was followed by an incubation at room temperature for 1h with ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).
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![Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245893)](https://www.abcam.com/ps/products/245/ab245893/Images/ab245893-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245893)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab245893 has not yet been referenced specifically in any publications.