Recombinant Alexa Fluor® 647 Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab310289)
![Alexa Fluor® 647 Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab310289)](https://www.abcam.com/ps/products/310/ab310289/Images/ab310289-1-MTOConjugatedRecombinantAntibodyBenefits.jpg "Alexa Fluor® 647 Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab310289)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR12722(B)] to DNA Polymerase beta
- Suitable for: Target binding affinity, Antibody labelling
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 647 Anti-DNA Polymerase beta antibody [EPR12722(B)]
See all DNA Polymerase beta primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EPR12722(B)] to DNA Polymerase beta -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: Target binding affinity, Antibody labellingmore details -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for Abtrial: learn more here.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12722(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-DNA Polymerase beta antibody [EPR12722(B)] - BSA and Azide free (ab249852)
- PE Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab306093)
- APC Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab306094)
- HRP Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab306095)
- AP Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab308977)
- Alexa Fluor® 555 Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab312277)
- Alexa Fluor® 568 Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab312771)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab310289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Target binding affinity |
Use at an assay dependent concentration.
|
|
| Antibody labelling |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Target binding affinity
Use at an assay dependent concentration. |
|
Antibody labelling
Use at an assay dependent concentration. |
Target
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Function
Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. -
Sequence similarities
Belongs to the DNA polymerase type-X family. -
Domain
Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity. -
Post-translational
modificationsMethylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity. -
Cellular localization
Nucleus, Cytoplasm - Information by UniProt
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Alternative names
- DNA directed DNA polymerase beta antibody
- DNA pol beta antibody
- DNA polymerase beta antibody
see all
Images
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Alexa Fluor® 647 Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab310289)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab310289 has not yet been referenced specifically in any publications.