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RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-Dopamine Transporter antibody [EPR19695] (ab300752)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Dopamine Transporter antibody [EPR19695] (AB300752)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Dopamine Transporter antibody [EPR19695] (AB300752)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Dopamine Transporter antibody [EPR19695] (AB300752)
  • Alexa Fluor® 647 Anti-Dopamine Transporter antibody [EPR19695] (AB300752)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR19695] to Dopamine Transporter
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Carrier Free Unconjugated

Overview

  • Product name

    Alexa Fluor® 647 Anti-Dopamine Transporter antibody [EPR19695]
    See all Dopamine Transporter primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR19695] to Dopamine Transporter

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Specificity

    Not suitable for IHC-FR in Rat

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse and rat striatum FFPE tissue sections. IHC-Fr: Mouse striatum fresh frozen tissue.

  • General notes

    Not suitable for IHC-FR in Rat

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab300752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/50.
Notes
IHC-P
1/100.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/50.

Target

  • Function

    Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.

  • Involvement in disease

    Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI) [MIM:613135]. It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs.

  • Sequence similarities

    Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • DA transporter antibody
      • DAT 1 antibody
      • DAT antibody
      • DAT1 antibody
      • Dopamine transporter 1 antibody
      • Dopamine transporter antibody
      • PKDYS antibody
      • SC6A3_HUMAN antibody
      • SLC6A3 antibody
      • Sodium dependent dopamine transporter antibody
      • Sodium-dependent dopamine transporter antibody
      • Solute carrier family 6 (neurotransmitter transporter dopamine), member 3 antibody
      • Solute carrier family 6 (neurotransmitter transporter), member 3 antibody
      • Solute carrier family 6 member 3 antibody
      • Variable number tandem repeat (VNTR) antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Dopamine Transporter antibody [EPR19695] (AB300752)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Dopamine Transporter antibody [EPR19695] (AB300752)

      Immunohistochemical analysis of paraffin-embedded mouse striatum tissue labeling Dopamine Transporter with ab300752 at 1/100 (5.0 µg/ml). Positive staining on mouse striatum. The section was incubated with ab300752 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Dopamine Transporter antibody [EPR19695] (AB300752)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Dopamine Transporter antibody [EPR19695] (AB300752)

      Immunohistochemical analysis of paraffin-embedded rat striatum tissue labeling Dopamine Transporter with ab300752 at 1/100 (5.0 µg/ml). Positive staining on rat striatum (shown in red). The section was incubated with ab300752 for 60 mins at room temperature. Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Dopamine Transporter antibody [EPR19695] (AB300752)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Dopamine Transporter antibody [EPR19695] (AB300752)

      Immunohistochemical analysis of 4% PFA-fixed / 0.2% Triton X-100 permeabilized frozen mouse striatum (fresh) tissue labeling Dopamine Transporter with ab300752 at 1/50 (10.0 µg/ml). Positive staining (Red) on mouse striatum . The nuclear counterstain was DAPI (Blue).

    • Alexa Fluor® 647 Anti-Dopamine Transporter antibody [EPR19695] (AB300752)
      Alexa Fluor® 647 Anti-Dopamine Transporter antibody [EPR19695] (AB300752)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab300752? Please let us know so that we can cite the reference in this datasheet.

    ab300752 has not yet been referenced specifically in any publications.

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