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Recombinant

Recombinant Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)

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Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
  • Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR1034Y] to GFAP
  • Suitable for: IHC-Fr, ICC/IF
  • Reacts with: Mouse, Rat
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Biotin Carrier Free HRP PE Unconjugated

Overview

  • Product name

    Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric
    See all GFAP primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR1034Y] to GFAP

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested applications

    Suitable for: IHC-Fr, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P Fr: Mouse cerebrum (fresh), mouse liver (fresh), rat cerebrum (fresh) and rat liver (fresh) tissue lysate. ICC/IF: Mouse and rat primary glia cell tissue lysate.

  • General notes

    ab313596 is a mouse monoclonal chimeric antibody.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab313596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
1/500.

Confocal image showing positive staining on mouse cerebrum. Low expression: confocal image showing no staining on mouse liver. Confocal image showing positive staining on rat cerebrum. Low expression: confocal image showing no staining on rat liver. 
ICC/IF
1/50.

Confocal image showing positive staining in mouse and rat primary glia cells.
Notes
IHC-Fr
1/500.

Confocal image showing positive staining on mouse cerebrum. Low expression: confocal image showing no staining on mouse liver. Confocal image showing positive staining on rat cerebrum. Low expression: confocal image showing no staining on rat liver. 
ICC/IF
1/50.

Confocal image showing positive staining in mouse and rat primary glia cells.

Target

  • Function

    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

  • Tissue specificity

    Expressed in cells lacking fibronectin.

  • Involvement in disease

    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Post-translational
    modifications

    Phosphorylated by PKN1.

  • Cellular localization

    Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt

  • Database links

    • Alternative names

      • wu:fb34h11 antibody
      • ALXDRD antibody
      • cb345 antibody
      • etID36982.3 antibody
      • FLJ42474 antibody
      • FLJ45472 antibody
      • GFAP antibody
      • GFAP_HUMAN antibody
      • gfapl antibody
      • Glial fibrillary acidic protein antibody
      • Intermediate filament protein antibody
      • wu:fk42c12 antibody
      • xx:af506734 antibody
      • zgc:110485 antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling GFAP with ab313596 at 1/500 (1.0 µ g/ml) dilution (Green). Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount ® .The immunostaining was performed on a Leica Biosystems BOND ® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling GFAP with ab313596 at 1/500 (1.0 µ g/ml) dilution (Green). Low expression: confocal image showing no staining on mouse liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount ® . The immunostaining was performed on a Leica Biosystems BOND ® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling GFAP with ab313596 at 1/500 (1.0 µ g/ml) dilution (Green). Confocal image showing positive staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount ® .The immunostaining was performed on a Leica Biosystems BOND ® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling GFAP with ab313596 at 1/500 (1.0 µ g/ml) dilution (Green). Low expression: confocal image showing no staining on rat liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount ® .The immunostaining was performed on a Leica Biosystems BOND ® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling GFAP with ab313596 at 1/50 (10.0 µ g/ml) dilution(Green). Confocal image showing positive staining in mouse primary glia cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Confocal scanning Z step was set as 0.3 µ m followed by image processing with maximum Z projection. ab194324 Anti-GFAP rabbit monoclonal antibody (Alexa Fluor ® 488) was used to counterstain tubulin at 1/100 5µ g/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling GFAP with ab313596 at 1/50 (10.0 µ g/ml) dilution(Green). Confocal image showing positive staining in rat primary glia cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Confocal scanning Z step was set as 0.3 µ m followed by image processing with maximum Z projection. ab194324 Anti-GFAP rabbit monoclonal antibody (Alexa Fluor ® 488) was used to counterstain tubulin at 1/100 5µ g/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    • Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
      Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab313596? Please let us know so that we can cite the reference in this datasheet.

    ab313596 has not yet been referenced specifically in any publications.

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