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RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-Huntingtin antibody [EPR5526] (ab196931)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Huntingtin antibody [EPR5526] (ab196931)
  • Alexa Fluor® 647 Anti-Huntingtin antibody [EPR5526] (ab196931)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR5526] to Huntingtin
  • Suitable for: ICC/IF
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Carrier Free Unconjugated

Overview

  • Product name

    Alexa Fluor® 647 Anti-Huntingtin antibody [EPR5526]
    See all Huntingtin primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR5526] to Huntingtin

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested applications

    Suitable for: ICC/IFmore details
    Unsuitable for: Flow Cyt (Intra)

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Neuro-2a cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5526

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab196931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
Notes
ICC/IF
1/100.
Application notes
Is unsuitable for Flow Cyt (Intra).

Target

  • Function

    May play a role in microtubule-mediated transport or vesicle function.

  • Tissue specificity

    Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.

  • Involvement in disease

    Defects in HTT are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum).

  • Sequence similarities

    Belongs to the huntingtin family.
    Contains 10 HEAT repeats.

  • Domain

    The N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations.

  • Post-translational
    modifications

    Cleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis.
    Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.

  • Cellular localization

    Cytoplasm. Nucleus. The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington's disease neurons.
  • Information by UniProt

  • Database links

    • Alternative names

      • AI256365 antibody
      • C430023I11Rik antibody
      • HD antibody
      • HD protein antibody
      • HD_HUMAN antibody
      • HDH antibody
      • HTT antibody
      • Huntingtin antibody
      • HUNTINGTON CHOREA antibody
      • Huntington disease protein antibody
      • Huntington's disease protein homolog antibody
      • IT 15 antibody
      • IT15 antibody
      • OTTMUSP00000026909 antibody
      • ZHD antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Huntingtin antibody [EPR5526] (ab196931)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Huntingtin antibody [EPR5526] (ab196931)

      ab196931 staining Huntingtin in Neuro-2a differentiated cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196931 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 647 Anti-Huntingtin antibody [EPR5526] (ab196931)
      Alexa Fluor® 647 Anti-Huntingtin antibody [EPR5526] (ab196931)

    References (0)

    Publishing research using ab196931? Please let us know so that we can cite the reference in this datasheet.

    ab196931 has not yet been referenced specifically in any publications.

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