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RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab210416)

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Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab210416)
  • Alexa Fluor® 647 Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab210416)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EP1353Y] to Importin 9/RANBP9
  • Suitable for: Flow Cyt (Intra)
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

Overview

  • Product name

    Alexa Fluor® 647 Anti-Importin 9/RANBP9 antibody [EP1353Y]
    See all Importin 9/RANBP9 primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EP1353Y] to Importin 9/RANBP9

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested applications

    Suitable for: Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt (intra): Neuro 2A cells (differentiated).

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab210416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.
Notes
Flow Cyt (Intra)
1/500.

Target

  • Function

    Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6.

  • Sequence similarities

    Belongs to the importin beta family.
    Contains 1 importin N-terminal domain.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Imp9 antibody
      • Imp9a antibody
      • Imp9b antibody
      • Importin-9 antibody
      • Importin-9a antibody
      • Ipo9 antibody
      • IPO9_HUMAN antibody
      • Ran-binding protein 9 antibody
      • RanBP9 antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab210416)
      Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab210416)

      Overlay histogram showing Neuro2A cells stained with ab210416 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210416, 1/500 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

      This antibody gave a positive signal in Neuro2A cells fixed with 4% formaldehyde (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

    • Alexa Fluor® 647 Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab210416)
      Alexa Fluor® 647 Anti-Importin 9/RANBP9 antibody [EP1353Y] (ab210416)

    References (0)

    Publishing research using ab210416? Please let us know so that we can cite the reference in this datasheet.

    ab210416 has not yet been referenced specifically in any publications.

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