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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194108)

Submit a review Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194108)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194108)
  • Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194108)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR8985(B)] to Lamin B1 - Nuclear Envelope Marker
  • Suitable for: ICC/IF
  • Knockout validated
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Carrier Free HRP Unconjugated

Overview

  • Product name

    Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker
    See all Lamin B1 primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR8985(B)] to Lamin B1 - Nuclear Envelope Marker

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells, HAP1-LMNB1 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
Notes
ICC/IF
1/100.

Target

  • Function

    Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

  • Involvement in disease

    Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Post-translational
    modifications

    B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.

  • Cellular localization

    Nucleus inner membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ADLD antibody
    • lamin B1 antibody
    • Lamin-B1 antibody
    • LMN antibody
    • LMN2 antibody
    • LMNB antibody
    • Lmnb1 antibody
    • LMNB1_HUMAN antibody
    • MGC111419 antibody
    • OTTHUMP00000159218 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194108)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194108)

    ab194108 staining Lamin B1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab194108 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194108)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194108)

    ab194108 staining Lamin B1 in wild-type HAP1 cells (top panel) and Lamin B1 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Tween20 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab194108 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194108)
    Alexa Fluor® 647 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194108)

References (1)

Publishing research using ab194108? Please let us know so that we can cite the reference in this datasheet.

ab194108 has been referenced in 1 publication.

  • Rashid R  et al. Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer. Sci Data 6:323 (2019). beta actin ; Human . PubMed: 31848351

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