Recombinant Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)] (ab200427)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)] (ab200427)](https://www.abcam.com/ps/products/200/ab200427/Images/ab200427-243001-anti-lamin-b2-antibody-epr9701b-alexa-fluor-647-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)] (ab200427)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR9701(B)] to Lamin B2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)]
See all Lamin B2 primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EPR9701(B)] to Lamin B2 -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: MCF7 cells IHC-P: Human bladder transitional cell carcinoma and cerebrum tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9701(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| ICC/IF | (1) |
1/200.
|
| Notes |
|---|
|
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
1/200. |
Target
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Function
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. -
Involvement in disease
Defects in LMNB2 are a cause of partial acquired lipodystrophy (APLD) [MIM:608709]. A rare childhood disease characterized by loss of subcutaneous fat from the face and trunk. Fat deposition on the pelvic girdle and lower limbs is normal or excessive. Most frequently, onset between 5 and 15 years of age. Most affected subjects are females and some show no other abnormality, but many develop glomerulonephritis, diabetes mellitus, hyperlipidemia, and complement deficiency. Mental retardation in some cases. APLD is a sporadic disorder of unknown etiology. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsB-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. -
Cellular localization
Nucleus inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 84823 Human
- Entrez Gene: 16907 Mouse
- Entrez Gene: 299625 Rat
- Omim: 150341 Human
- SwissProt: Q03252 Human
- SwissProt: P21619 Mouse
- Unigene: 728836 Human
- Unigene: 7362 Mouse
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Alternative names
- LAMB 2 antibody
- LAMB2 antibody
- Lamin-B2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)] (ab200427)
ab200427 staining Lamin B2 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200427 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)] (ab200427)](https://www.abcam.com/ps/products/200/ab200427/Images/ab200427-490024-alexa-fluor-647-anti-lamin-b2-antibody-epr9701-B-immunohistochemistry-human-bladder.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)] (ab200427)Immunohistochemistry analysis of paraffin-embedded human bladder transitional cell carcinoma tissue section labelling Lamin B2 with ab200427 at 1/50 dilution. The section was incubated with ab200427 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins.
Nuclear envelope staining on human bladder transitional cell carcinoma tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Negative control: Phosphate buffered saline (PBS).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)] (ab200427)](https://www.abcam.com/ps/products/200/ab200427/Images/ab200427-490025-alexa-fluor-647-anti-lamin-b2-antibody-epr9701-B-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)] (ab200427)Immunohistochemistry analysis of paraffin-embedded human cerebrum tissue section labelling Lamin B2 with ab200427 at 1/50 dilution. The section was incubated with ab200427 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins.
Nuclear envelope staining on human cerebrum tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Negative control: Phosphate buffered saline (PBS).
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![Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)] (ab200427)](https://www.abcam.com/ps/products/200/ab200427/Images/ab200427-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-Lamin B2 antibody [EPR9701(B)] (ab200427)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab200427 has been referenced in 1 publication.
- Burlingame EA et al. Toward reproducible, scalable, and robust data analysis across multiplex tissue imaging platforms. Cell Rep Methods 1:N/A (2021). PubMed: 34485971