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  1. Link

    products/primary-antibodies/alexa-fluor-647-neun-antibody-epr12763-neuronal-marker-ab190565.pdf

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Neuroscience Cell Type Marker Neuron marker Soma marker
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RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

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  • SDS
Reviews (7)Q&A (1)References (12)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
  • Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker
  • Suitable for: IHC-P, ICC/IF, IHC-Fr
  • Reacts with: Mouse, Rat, Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Biotin Carrier Free FITC PE Unconjugated

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Alexa Fluor® 405 Anti-GFAP antibody [EPR1034Y] (ab206586)
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Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (ab269823)

View more associated products

Overview

  • Product name

    Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker
    See all NeuN primary antibodies
  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NeuN aa 1-100 (Cysteine residue). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: A6NFN3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Mouse, rat and human cerebellum tissue; IHC-Fr: Mouse and Rat cerebellum; ICC/IF: NGF-differentiated PC12 and U87-MG cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12763
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Neuronal
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Other FOXes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Alternative Versions

    • Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    • Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190195)
    • Biotin Anti-NeuN antibody [EPR12763] (ab204681)
    • Alexa Fluor® 594 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207279)
    • Alexa Fluor® 555 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207281)
    • Alexa Fluor® 568 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207282)
    • Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)
    • FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
  • Isotype control

    • Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab190565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
1/50.

ab199093 - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control with this antibody.

ICC/IF
1/50.
IHC-Fr (1)
1/1000.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Notes
IHC-P
1/50.

ab199093 - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control with this antibody.

ICC/IF
1/50.
IHC-Fr
1/1000.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Target

  • Function

    RNA-binding protein that regulates alternative splicing events.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Target information above from: UniProt accession A6NFN3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 146713 Human
    • Entrez Gene: 52897 Mouse
    • Entrez Gene: 287847 Rat
    • SwissProt: A6NFN3 Human
    • SwissProt: Q8BIF2 Mouse
    • Unigene: 135229 Human
    • Unigene: 341103 Mouse
    • Alternative names

      • FLJ56884 antibody
      • FLJ58356 antibody
      • Fox-1 homolog C antibody
      • fox1 homolog C antibody
      • Fox3 antibody
      • FOX3NeuN antibody
      • hexaribonucleotide binding protein 3 antibody
      • HRNBP3 antibody
      • NEUN antibody
      • neuronal nuclei antibody
      • Rbfox3 antibody
      • RFOX3_HUMAN antibody
      • RNA binding protein fox-1 homolog 3 antibody
      • RNA binding protein, fox 1 homolog (C. elegans) 3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

      IHC image of ab190565 staining in formalin fixed paraffin embedded tissue section of normal human cerebellum.

      The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with ab190565 (1/50) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then counterstained and mounted with SlowFade® Gold Antifade Mountant with DAPI.

      The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is dervied directly from bound ab190565. The separate images of ab190565 and DAPI alone, combined with the merged version of both signals, shows predominant co-localisation of the Alexa Fluor® 647 signal in the nuclei of the cerebellar granule layer.

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
      Immunohistochemistry (Frozen sections) analysis of mouse cerebellum tissue sections labeling NeuN with Purified ab190565 at 1/1000 (2.0 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

      ab190565 staining NeuN in U87-MG cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190565 at 1/50 dilution(shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Alexa Fluor® 488 Goat anti-Mouse secondary (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
      Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
      Immunohistochemistry (Frozen sections) analysis of rat cerebellum tissue sections labeling NeuN with Purified ab190565 at 1/1000 (2.0 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)He WM et al ci Rep. 2019 Feb 19;9(1):2287. doi: 10.1038/s41598-019-38750-0. Fig 5a. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Effects of delayed treatment of L655,708 on neurogenesis in the peri-infarct region. Ki67, a proliferative marker, and NeuN, a neuronal marker, were used to evaluate neurogenesis in the peri-infarct region. The nuclei were counter-stained with DAPI. Asterisk represents the significant difference from vehicle (n = 8/group; p < 0.05). Pond represents the significant difference from 1 mg group (n = 8/group; p < 0.05). Scale bar = 25 µm.

      Panel A shown only. Rat brain sections were first pre-treated with citrate buffer (0.01 mol/L, pH 6.0) for 5 minutes at 85 °C, followed by incubation with 5% normal goat serum for 1 hour at room temperature. Sections were then incubated with anti-Ki67 (rabbit monoclonal antibody to Ki67, 1/500; ab16667) and anti-NeuN (rabbit monoclonal antibody to NeuN, 1/500; ab190565), overnight at 4°C. Sections were then rinsed in phosphate-buffered saline 3 times, followed by incubation with rabbit secondary antibody (anti-rabbit IgG, 1/1000) for 1 hour at room temperature. Fluorescence signals were then detected under a microscope. Negative control was conducted by incubating sections with PBS instead of primary antibodies. No positive signals were shown in negative control.

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

      ab190565 staining NeuN in NGF-differentiated PC12 cells (7 days). The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190565 at 1/50 dilution (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Alexa Fluor® 488 Goat anti-Mouse secondary (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

      IHC image of ab190565 staining in formalin fixed paraffin embedded tissue section of normal rat cerebellum.

      The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with ab190565 (1/50) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then counterstained and mounted with SlowFade® Gold Antifade Mountant with DAPI.

      The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is dervied directly from bound ab190565. The separate images of ab190565 and DAPI alone, combined with the merged version of both signals, shows predominant co-localisation of the Alexa Fluor® 647 signal in the nuclei of the cerebellar granule layer.

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

      IHC image of ab190565 staining in formalin fixed paraffin embedded tissue section of normal mouse cerebellum.

      The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with ab190565 (1/50) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then counterstained and mounted with SlowFade® Gold Antifade Mountant with DAPI.

      The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is dervied directly from bound ab190565. The separate images of ab190565 and DAPI alone, combined with the merged version of both signals, shows predominant co-localisation of the Alexa Fluor® 647 signal in the nuclei of the cerebellar granule layer.

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    • Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
      Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (12)

    Publishing research using ab190565? Please let us know so that we can cite the reference in this datasheet.

    ab190565 has been referenced in 12 publications.

    • Mou H  et al. Airway basal stem cells generate distinct subpopulations of PNECs. Cell Rep 35:109011 (2021). PubMed: 33882306
    • Gonzalez-Fernandez E  et al. 20-HETE Enzymes and Receptors in the Neurovascular Unit: Implications in Cerebrovascular Disease. Front Neurol 11:983 (2020). PubMed: 33013649
    • Lin G  et al. Involvement of the Nrf2-Keap1 signaling pathway in protection against thallium-induced oxidative stress and mitochondrial dysfunction in primary hippocampal neurons. Toxicol Lett 319:66-73 (2020). PubMed: 31726083
    • Zayas-Santiago A  et al. Accumulation of amyloid beta in human glioblastomas. Clin Exp Immunol 202:325-334 (2020). PubMed: 32654112
    • Kalafateli AL  et al. An amylin and calcitonin receptor agonist modulates alcohol behaviors by acting on reward-related areas in the brain. Prog Neurobiol N/A:101969 (2020). PubMed: 33278524
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Frozen sections) abreview for Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Shaogen Wu

    Verified customer

    Submitted Apr 06 2022

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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