For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/primary-antibodies/alexa-fluor-647-neuropilin-1-antibody-epr3113-ab198323.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Angiogenesis Growth Factors VEGF VEGF Receptors
Share by email
RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-Neuropilin 1 antibody [EPR3113] (ab198323)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Neuropilin 1 antibody [EPR3113] (ab198323)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Neuropilin 1 antibody [EPR3113] (ab198323)
  • Alexa Fluor® 647 Anti-Neuropilin 1 antibody [EPR3113] (ab198323)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR3113] to Neuropilin 1
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free PE Unconjugated

You may also be interested in

Primary
Product image
Alexa Fluor® 488 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab196376)
Primary
Product image
Alexa Fluor® 488 Anti-Neuropilin 1 antibody [EPR3113] (ab197644)
Knockout
Product image
Human NRP1 knockout A549 cell line (ab269507)

View more associated products

Overview

  • Product name

    Alexa Fluor® 647 Anti-Neuropilin 1 antibody [EPR3113]
    See all Neuropilin 1 primary antibodies
  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR3113] to Neuropilin 1
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab189308)

  • Positive control

    • ICC/IF: HeLa cells. Flow Cyt (intra): HepG2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3113
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • VEGF
    • VEGF Receptors
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
    • Alexa Fluor® 488 Anti-Neuropilin 1 antibody [EPR3113] (ab197644)
    • PE Anti-Neuropilin 1 antibody [EPR3113] (ab209445)
    • Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Immunizing Peptide (Blocking)

    • Neuropilin 1 peptide (ab189308)
  • Isotype control

    • Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093)
  • Recombinant Protein

    • Recombinant human Neuropilin 1 protein (Fc Chimera Active) (ab221023)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab198323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
Flow Cyt (Intra)
1/50.

ab199093 - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control with this antibody.

Notes
ICC/IF
1/100.
Flow Cyt (Intra)
1/50.

ab199093 - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control with this antibody.

Target

  • Function

    The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
    The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
  • Tissue specificity

    The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
  • Sequence similarities

    Belongs to the neuropilin family.
    Contains 2 CUB domains.
    Contains 2 F5/8 type C domains.
    Contains 1 MAM domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession O14786 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8829 Human
    • Omim: 602069 Human
    • SwissProt: O14786 Human
    • Unigene: 131704 Human
    • Unigene: 653996 Human
    • Alternative names

      • A5 protein antibody
      • BDCA4 antibody
      • BLOOD DENDRITIC CELL ANTIGEN 4 antibody
      • CD304 antibody
      • Neuropilin-1 antibody
      • Neuropilin1 antibody
      • NP1 antibody
      • NPN1 antibody
      • NRP 1 antibody
      • NRP antibody
      • NRP1 antibody
      • NRP1_HUMAN antibody
      • transmembrane receptor antibody
      • Vascular endothelial cell growth factor 165 receptor antibody
      • VEGF165R antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Neuropilin 1 antibody [EPR3113] (ab198323)
      Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Neuropilin 1 antibody [EPR3113] (ab198323)

      Overlay histogram showing HepG2 cells stained with ab198323 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab198323, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Neuropilin 1 antibody [EPR3113] (ab198323)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Neuropilin 1 antibody [EPR3113] (ab198323)

      ab198323 staining Neuropilin 1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab198323 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 647 Anti-Neuropilin 1 antibody [EPR3113] (ab198323)
      Alexa Fluor® 647 Anti-Neuropilin 1 antibody [EPR3113] (ab198323)

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab198323? Please let us know so that we can cite the reference in this datasheet.

    ab198323 has been referenced in 1 publication.

    • Al-Zeheimi N & Adham SA Modeling Neoadjuvant chemotherapy resistance in vitro increased NRP-1 and HER2 expression and converted MCF7 breast cancer subtype. Br J Pharmacol 177:2024-2041 (2020). PubMed: 31883395

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab198323.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.