Recombinant Alexa Fluor® 647 Anti-p75 NGF Receptor antibody [EP1039Y] (ab195180)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-p75 NGF Receptor antibody [EP1039Y] (ab195180)](https://www.abcam.com/ps/products/195/ab195180/Images/ab195180-237416-anti-p75-ngf-receptor-antibody-ep1039y-alexa-fluor-647-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-p75 NGF Receptor antibody [EP1039Y] (ab195180)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EP1039Y] to p75 NGF Receptor
- Suitable for: ICC/IF
- Reacts with: Rat
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
Overview
-
Product name
Alexa Fluor® 647 Anti-p75 NGF Receptor antibody [EP1039Y]
See all p75 NGF Receptor primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EP1039Y] to p75 NGF Receptor -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: PC12 cells
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 3.25 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1039Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
|
| Notes |
|---|
|
ICC/IF
1/100. |
Target
-
Function
Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. -
Sequence similarities
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
Domain
Death domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1. -
Post-translational
modificationsN- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 24596 Rat
- SwissProt: P07174 Rat
- Unigene: 10980 Rat
-
Alternative names
- CD271 antibody
- CD271 antigen antibody
- Gp80 LNGFR antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-p75 NGF Receptor antibody [EP1039Y] (ab195180)
ab195180 staining p75 NGF receptor in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195180 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
This product gave a positive signal in 4% formaldehyde (10 min) fixed PC12 cells under the same testing conditions.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
-
![Alexa Fluor® 647 Anti-p75 NGF Receptor antibody [EP1039Y] (ab195180)](https://www.abcam.com/ps/products/195/ab195180/Images/ab195180-2-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-p75 NGF Receptor antibody [EP1039Y] (ab195180)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab195180 has been referenced in 5 publications.
- Eng J et al. Cyclic Multiplexed-Immunofluorescence (cmIF), a Highly Multiplexed Method for Single-Cell Analysis. Methods Mol Biol 2055:521-562 (2020). PubMed: 31502168
- Pereiro X et al. Comparative lipidomic analysis of mammalian retinal ganglion cells and Müller glia in situ and in vitro using High-Resolution Imaging Mass Spectrometry. Sci Rep 10:20053 (2020). PubMed: 33208898
- Lin JR et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7:N/A (2018). PubMed: 29993362
- Lin JR et al. Cyclic Immunofluorescence (CycIF), A Highly Multiplexed Method for Single-cell Imaging. Curr Protoc Chem Biol 8:251-264 (2016). PubMed: 27925668
- Lin JR et al. Highly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence method. Nat Commun 6:8390 (2015). PubMed: 26399630