Recombinant Alexa Fluor® 647 Anti-PAX8 antibody [EPR18715] (ab215953)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PAX8 antibody [EPR18715] (ab215953)](https://www.abcam.com/ps/products/215/ab215953/Images/ab215953-275446-anti-pax8-antibody-epr18715-alexa-fluor-647-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PAX8 antibody [EPR18715] (ab215953)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR18715] to PAX8
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 647 Anti-PAX8 antibody [EPR18715]
See all PAX8 primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EPR18715] to PAX8 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human PAX8 aa 100 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q06710 -
Positive control
- ICC/IF: OVCAR-3 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18715 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/200.
This product gave a positive signal in OVCAR-3 cells fixed with 4% formaldehyde (10 min) |
| Notes |
|---|
|
ICC/IF
1/200. This product gave a positive signal in OVCAR-3 cells fixed with 4% formaldehyde (10 min) |
Target
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Function
Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells. -
Tissue specificity
Expressed in the excretory system, thyroid gland and Wilms tumors. -
Involvement in disease
Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue. -
Sequence similarities
Contains 1 paired domain. -
Developmental stage
In developing excretory system, during thyroid differentiation and in adult thyroid. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7849 Human
- Omim: 167415 Human
- SwissProt: Q06710 Human
- Unigene: 469728 Human
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Alternative names
- OTTHUMP00000158659 antibody
- OTTHUMP00000158660 antibody
- OTTHUMP00000203723 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PAX8 antibody [EPR18715] (ab215953)
ab215953 staining PAX8 in OVCAR-3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.
The cells were then incubated overnight at +4°C with ab215953 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Alexa Fluor® 647 Anti-PAX8 antibody [EPR18715] (ab215953)](https://www.abcam.com/ps/products/215/ab215953/Images/ab215953-2-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-PAX8 antibody [EPR18715] (ab215953)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab215953 has been referenced in 2 publications.
- Capellero S et al. Ovarian Cancer Cells in Ascites Form Aggregates That Display a Hybrid Epithelial-Mesenchymal Phenotype and Allows Survival and Proliferation of Metastasizing Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35055018
- Lin JR et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7:N/A (2018). PubMed: 29993362