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RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175)

Reviews (1) Submit a question References (4)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175)
  • Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EP1576Y] to S100 beta - Astrocyte Marker
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 594 Unconjugated

Overview

  • Product name

    Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker
    See all S100 beta primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EP1576Y] to S100 beta - Astrocyte Marker

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Goat, Zebrafish, Macaque monkey

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: A375 cells, Malme-3M cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab196175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/50.

This product gave a positive signal in A375 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).
Notes
ICC/IF
1/50.

This product gave a positive signal in A375 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Target

  • Function

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.

  • Tissue specificity

    Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

  • Sequence similarities

    Belongs to the S-101 family.
    Contains 2 EF-hand domains.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • NEF antibody
    • Protein S100 B antibody
    • Protein S100-B antibody
    • S 100 calcium binding protein beta chain antibody
    • S 100 protein beta chain antibody
    • S-100 protein beta chain antibody
    • S-100 protein subunit beta antibody
    • S100 antibody
    • S100 calcium binding protein beta (neural) antibody
    • S100 calcium-binding protein B antibody
    • S100 protein beta chain antibody
    • S100B antibody
    • S100B_HUMAN antibody
    • S100beta antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175)

    ab196175 staining S100 beta in Malme-3M cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196175 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175)

    ab196175 staining S100 beta in A375 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196175 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175)
    Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab196175)

References (4)

Publishing research using ab196175? Please let us know so that we can cite the reference in this datasheet.

ab196175 has been referenced in 4 publications.

  • Willows JW  et al. A clearing-free protocol for imaging intact whole adipose tissue innervation in mice. STAR Protoc 3:101109 (2022). PubMed: 35106499
  • Menz J  et al. Increased mortality and altered local immune response in secondary peritonitis after previous visceral operations in mice. Sci Rep 11:16175 (2021). PubMed: 34376743
  • Fraser J  et al. Common Regulatory Targets of NFIA, NFIX and NFIB during Postnatal Cerebellar Development. Cerebellum 19:89-101 (2020). PubMed: 31838646
  • Shohayeb B  et al. The Spindle-Associated Microcephaly Protein, WDR62, Is Required for Neurogenesis and Development of the Hippocampus. Front Cell Dev Biol 8:549353 (2020). PubMed: 33042990

Customer reviews and Q&As

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Flow Cytometry abreview for Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker

 Good
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (iPSC-derived astrocytes)
Permeabilization
Yes - Cytoperm buffer from BD Biosciences (cat: 554722)
Gating Strategy
First, live cell population was gated on. Then, AF647 positive cells were detected.
Specification
iPSC-derived astrocytes
Preparation
Cell harvesting/tissue preparation method: The cells were harvested using TrypLE, resuspended in FACS buffer. The cells suspension is distributed in round bottom 96-well plate as 200 000 cells/well.
Sample buffer: FACS buffer (0.1%NaN3, 2.5% fetal calf serum in PBS)
Fixation
Cytofix buffer from BD Biosciences (cat: 554722)
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Apr 20 2022

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