RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-Sumo 1 antibody [Y299] (ab196533)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Sumo 1 antibody [Y299] (ab196533)
  • Alexa Fluor® 647 Anti-Sumo 1 antibody [Y299] (ab196533)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [Y299] to Sumo 1
  • Suitable for: ICC/IF
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alkaline Phosphatase APC Carrier Free HRP PE Unconjugated

Overview

  • Product name

    Alexa Fluor® 647 Anti-Sumo 1 antibody [Y299]
    See all Sumo 1 primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [Y299] to Sumo 1

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: NIH3T3 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab196533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/50.
Notes
ICC/IF
1/50.

Target

  • Function

    Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.

  • Involvement in disease

    Defects in SUMO1 are the cause of non-syndromic orofacial cleft type 10 (OFC10) [MIM:613705]; also called non-syndromic cleft lip with or without cleft palate 10. OFC10 is a birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. Note=A chromosomal aberation involving SUMO1 is the cause of OFC10. Translocation t(2;8)(q33.1;q24.3). The breakpoint occurred in the SUMO1 gene and resulted in haploinsufficiency confirmed by protein assays.

  • Sequence similarities

    Belongs to the ubiquitin family. SUMO subfamily.
    Contains 1 ubiquitin-like domain.

  • Post-translational
    modifications

    Cleavage of precursor form by SENP1 or SENP2 is necessary for function.
    Polymeric SUMO1 chains undergo polyubiquitination by RNF4.

  • Cellular localization

    Nucleus membrane. Nucleus speckle. Cytoplasm. Recruited by BCL11A into the nuclear body.
  • Information by UniProt

  • Database links

    • Alternative names

      • DAP1 antibody
      • GAP modifying protein 1 antibody
      • GAP-modifying protein 1 antibody
      • GMP 1 antibody
      • GMP1 antibody
      • OFC10 antibody
      • PIC 1 antibody
      • PIC1 antibody
      • SENP2 antibody
      • Sentrin 1 antibody
      • Sentrin antibody
      • Small ubiquitin related modifier 1 antibody
      • Small ubiquitin-like modifier 1 antibody
      • Small ubiquitin-related modifier 1 antibody
      • SMT3 antibody
      • SMT3 homolog 3 antibody
      • SMT3 suppressor of mif two 3 homolog 1 antibody
      • SMT3, yeast, homolog 3 antibody
      • Smt3C antibody
      • SMT3H3 antibody
      • SUMO-1 antibody
      • SUMO1 antibody
      • SUMO1_HUMAN antibody
      • Ubiquitin homology domain protein PIC1 antibody
      • Ubiquitin Like 1 antibody
      • Ubiquitin like protein SMT3C antibody
      • Ubiquitin like protein UBL1 antibody
      • Ubiquitin-homology domain protein PIC1 antibody
      • Ubiquitin-like protein SMT3C antibody
      • Ubiquitin-like protein UBL1 antibody
      • UBL 1 antibody
      • UBL1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Sumo 1 antibody [Y299] (ab196533)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Sumo 1 antibody [Y299] (ab196533)

      ab196533 staining Sumo 1 in NIH3T3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196533 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

      This product gave a positive signal in 100% methanol (5 min) fixed NIH3T3 cells under the same testing conditions.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 647 Anti-Sumo 1 antibody [Y299] (ab196533)
      Alexa Fluor® 647 Anti-Sumo 1 antibody [Y299] (ab196533)

    References (0)

    Publishing research using ab196533? Please let us know so that we can cite the reference in this datasheet.

    ab196533 has not yet been referenced specifically in any publications.

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