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RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-TLE 1 antibody [EPR9386(2)] (ab198525)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TLE 1 antibody [EPR9386(2)] (ab198525)
  • Alexa Fluor® 647 Anti-TLE 1 antibody [EPR9386(2)] (ab198525)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR9386(2)] to TLE 1
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free Unconjugated

Overview

  • Product name

    Alexa Fluor® 647 Anti-TLE 1 antibody [EPR9386(2)]
    See all TLE 1 primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR9386(2)] to TLE 1

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9386(2)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab198525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).
Notes
ICC/IF
1/100.

This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Target

  • Function

    Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.

  • Tissue specificity

    In all tissues examined, mostly in brain, liver and muscle.

  • Sequence similarities

    Belongs to the WD repeat Groucho/TLE family.
    Contains 6 WD repeats.

  • Domain

    WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain.

  • Post-translational
    modifications

    Phosphorylated, probably by CDK1. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1.
    Ubiquitinated by XIAP/BIRC4.

  • Cellular localization

    Nucleus. Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • E(Sp1) homolog antibody
    • Enhancer of split groucho 1 antibody
    • Enhancer of split groucho like protein 1 antibody
    • Enhancer of split groucho-like protein 1 antibody
    • ESG antibody
    • ESG1 antibody
    • GRG1 antibody
    • TLE1 antibody
    • TLE1_HUMAN antibody
    • Transducin like enhancer of split 1 (E(sp1) homolog Drosophila) antibody
    • Transducin like enhancer of split 1 antibody
    • Transducin like enhancer of split 1 homolog of Drosophila E(sp1) antibody
    • Transducin like enhancer protein 1 antibody
    • Transducin-like enhancer protein 1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TLE 1 antibody [EPR9386(2)] (ab198525)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TLE 1 antibody [EPR9386(2)] (ab198525)

    ab198525 staining TLE 1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198525 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 100% methanol (5min).

  • Alexa Fluor® 647 Anti-TLE 1 antibody [EPR9386(2)] (ab198525)
    Alexa Fluor® 647 Anti-TLE 1 antibody [EPR9386(2)] (ab198525)

References (0)

Publishing research using ab198525? Please let us know so that we can cite the reference in this datasheet.

ab198525 has not yet been referenced specifically in any publications.

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