Recombinant Alexa Fluor® 647 Anti-TMEM119 antibody [106-6] (ab225494)
![Flow Cytometry - Alexa Fluor® 647 Anti-TMEM119 antibody [106-6] (ab225494)](https://www.abcam.com/ps/products/225/ab225494/Images/ab225494-331308-anti-tmem119-antibody-106-6-alexa-fluor-647-flow-cytometry.jpg "Flow Cytometry - Alexa Fluor® 647 Anti-TMEM119 antibody [106-6] (ab225494)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [106-6] to TMEM119
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 647 Anti-TMEM119 antibody [106-6]
See all TMEM119 primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [106-6] to TMEM119 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment (MBP-tag) within Mouse TMEM119 aa 1-100 (extracellular). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8R138 -
Positive control
- Flow Cyt: HEK-293T transfected with Myc-His tagged TMEM119 expression vector.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
106-6 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (1) |
1/500.
The cellular localisation of this product has been verified in ICC/IF. |
| Notes |
|---|
|
Flow Cyt
1/500. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Cellular localization
Membrane; Single-pass type I membrane protein -
Database links
- Entrez Gene: 338773 Human
- Entrez Gene: 231633 Mouse
- SwissProt: Q4V9L6 Human
- SwissProt: Q8R138 Mouse
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Alternative names
- OBIF antibody
- Osteoblast induction factor antibody
- PSEC0199 antibody
see all
Images
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Flow Cytometry - Alexa Fluor® 647 Anti-TMEM119 antibody [106-6] (ab225494)
Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225494 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Alexa Fluor® 647 ab199093 (left). Cells were surface-stained with ab225494, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 488 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.
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![Alexa Fluor® 647 Anti-TMEM119 antibody [106-6] (ab225494)](https://www.abcam.com/ps/products/225/ab225494/Images/ab225494-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-TMEM119 antibody [106-6] (ab225494)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab225494 has been referenced in 1 publication.
- Ruhela D et al. Astrocyte-derived microparticles initiate a neuroinflammatory cycle due to carbon monoxide poisoning. Brain Behav Immun Health 18:100398 (2021). PubMed: 34917988