Recombinant Alexa Fluor® 647 Anti-Topoisomerase I antibody [EPR5375] (ab196930)
![Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Topoisomerase I antibody [EPR5375] (ab196930)](https://www.abcam.com/ps/products/196/ab196930/Images/ab196930-239603-anti-topoisomerase-i-antibody-epr5375-alexa-fluor-647-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Topoisomerase I antibody [EPR5375] (ab196930)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR5375] to Topoisomerase I
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 647 Anti-Topoisomerase I antibody [EPR5375]
See all Topoisomerase I primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EPR5375] to Topoisomerase I -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
Does not react with:
Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: T47D cells. Flow Cyt (intra): T47D cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5375 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
This product gave a positive signal in T47D cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) |
|
| Flow Cyt (Intra) |
1/500.
ab199093 - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC/IF
1/100. This product gave a positive signal in T47D cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) |
|
Flow Cyt (Intra)
1/500. ab199093 - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control with this antibody. |
Target
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Function
The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. -
Involvement in disease
Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. -
Sequence similarities
Belongs to the eukaryotic type I topoisomerase family. -
Post-translational
modificationsSumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment. -
Cellular localization
Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli. - Information by UniProt
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Database links
- Entrez Gene: 7150 Human
- Entrez Gene: 21969 Mouse
- Omim: 126420 Human
- SwissProt: P11387 Human
- SwissProt: Q04750 Mouse
- Unigene: 472737 Human
- Unigene: 712686 Human
- Unigene: 217233 Mouse
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Alternative names
- DNA topoisomerase 1 antibody
- DNA topoisomerase I antibody
- NUP98 fusion gene antibody
see all
Images
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Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Topoisomerase I antibody [EPR5375] (ab196930)
Overlay histogram showing T47D cells stained with ab196930 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196930, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.
This antibody gave a positive signal in T47D fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Topoisomerase I antibody [EPR5375] (ab196930)](https://www.abcam.com/ps/products/196/ab196930/Images/ab196930-237323-anti-topoisomerase-i-antibody-epr5375-alexa-fluor-647-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Topoisomerase I antibody [EPR5375] (ab196930)ab196930 staining Topoisomerase I in T47D cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196930 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).
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![Alexa Fluor® 647 Anti-Topoisomerase I antibody [EPR5375] (ab196930)](https://www.abcam.com/ps/products/196/ab196930/Images/ab196930-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-Topoisomerase I antibody [EPR5375] (ab196930)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab196930 has not yet been referenced specifically in any publications.