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    products/primary-antibodies/alexa-fluor-680-beta-actin-antibody-mabcam-8226-loading-control-ab184092.pdf

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Isotype/Loading Controls Loading Controls Beta Actin
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Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184092)

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Western blot - Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184092)

    Key features and details

    • Alexa Fluor® 680 Mouse monoclonal [mAbcam 8226] to beta Actin - Loading Control
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Conjugation: Alexa Fluor® 680. Ex: 679nm, Em: 702nm
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Carrier Free Unconjugated

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    Overview

    • Product name

      Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control
      See all beta Actin primary antibodies
    • Description

      Alexa Fluor® 680 Mouse monoclonal [mAbcam 8226] to beta Actin - Loading Control
    • Host species

      Mouse
    • Conjugation

      Alexa Fluor® 680. Ex: 679nm, Em: 702nm
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Human
    • Immunogen

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • Positive control

      • This antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293; Jurkat; NIH3T3.
    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Clonality

      Monoclonal
    • Clone number

      mAbcam 8226
    • Myeloma

      Sp2/0-Ag14
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Isotype/Loading Controls
      • Loading Controls
      • Beta Actin
      • Tags & Cell Markers
      • Subcellular Markers
      • Cytoskeleton
      • Actin
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Actin

    Associated products

    • Alternative Versions

      • Alexa Fluor® 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184576)
      • HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)
      • Anti-beta Actin antibody [mAbcam 8226] - BSA and Azide free (ab264083)
      • Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)
    • Recombinant Protein

      • Recombinant Human beta Actin protein (His tag) (ab240844)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab184092 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

    Target

    • Function

      Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in disease

      Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
    • Sequence similarities

      Belongs to the actin family.
    • Post-translational
      modifications

      ISGylated.
    • Cellular localization

      Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Target information above from: UniProt accession P60709 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 60 Human
      • Entrez Gene: 11461 Mouse
      • Omim: 102630 Human
      • SwissProt: P60709 Human
      • SwissProt: P60710 Mouse
      • Unigene: 520640 Human
      • Unigene: 708120 Human
      • Unigene: 727576 Human
      • Unigene: 328431 Mouse
      • Unigene: 391967 Mouse
      see all
    • Alternative names

      • A26C1A antibody
      • A26C1B antibody
      • ACTB antibody
      • ACTB_HUMAN antibody
      • Actin beta antibody
      • Actin cytoplasmic 1 antibody
      • Actin, cytoplasmic 1, N-terminally processed antibody
      • Actx antibody
      • b actin antibody
      • b-actin antibody
      • Beta cytoskeletal actin antibody
      • Beta-actin antibody
      • BRWS1 antibody
      • E430023M04Rik antibody
      • Melanoma X actin antibody
      • MGC128179 antibody
      • PS1TP5 binding protein 1 antibody
      • PS1TP5BP1 antibody
      see all

    Images

    • Western blot - Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184092)
      Western blot - Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184092)
      All lanes : Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184092) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 42 kDa
      Observed band size: 42 kDa



      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab184092 overnight at 4°C. Antibody binding was detected after washing to remove excess antibody and imaged using the Licor Odyssey CLx.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab184092? Please let us know so that we can cite the reference in this datasheet.

    ab184092 has not yet been referenced specifically in any publications.

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