Anti-ALIX antibody (ab88743)
Key features and details
- Mouse polyclonal to ALIX
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ALIX antibody
See all ALIX primary antibodies -
Description
Mouse polyclonal to ALIX -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human ALIX. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: lysate from Human liver or HeLa cells; ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. |
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ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation. -
Sequence similarities
Contains 1 BRO1 domain. -
Cellular localization
Cytoplasm > cytosol. Melanosome. Cytoplasm > cytoskeleton > centrosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells. - Information by UniProt
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Database links
- Entrez Gene: 10015 Human
- Omim: 608074 Human
- SwissProt: Q8WUM4 Human
- Unigene: 475896 Human
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Alternative names
- AIP1 antibody
- ALG 2 interacting protein 1 antibody
- ALG-2-interacting protein 1 antibody
see all
Images
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Western blot - Anti-ALIX antibody (ab88743)Anti-ALIX antibody (ab88743) at 1 µg/ml + Human liver lysate at 50 µg
Predicted band size: 97 kDa -
Western blot - Anti-ALIX antibody (ab88743)Anti-ALIX antibody (ab88743) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 97 kDa -
Western blot - Anti-ALIX antibody (ab88743)All lanes : Anti-ALIX antibody (ab88743) at 1 µg/ml
Lane 1 : PDC6I-transfected 293T cell lysate
Lane 2 : non transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 97 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-ALIX antibody (ab88743)Immunofluorescence analysis of PDC6I expression in PFA-fixed, permeabilized HeLa cells using ab88743 at 10 µg/ml, followed by a fluorescein-conjugated secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab88743 has been referenced in 19 publications.
- Ma L et al. Neural Stem Cell-Derived Exosomal Netrin1 Contributes to Neuron Differentiation of Mesenchymal Stem Cells in Therapy of Spinal Bifida Aperta. Stem Cells Transl Med 11:539-551 (2022). PubMed: 35325230
- Li N & Wang B Suppressive effects of umbilical cord mesenchymal stem cell-derived exosomal miR-15a-5p on the progression of cholangiocarcinoma by inhibiting CHEK1 expression. Cell Death Discov 8:205 (2022). PubMed: 35428780
- Xu M et al. Tumor associated macrophages-derived exosomes facilitate hepatocellular carcinoma malignance by transferring lncMMPA to tumor cells and activating glycolysis pathway. J Exp Clin Cancer Res 41:253 (2022). PubMed: 35986343
- Du L et al. Human umbilical cord mesenchymal stem cell-derived exosomes inhibit migration and invasion of breast cancer cells via miR-21-5p/ZNF367 pathway. Breast Cancer 28:829-837 (2021). PubMed: 33770377
- Meng Q et al. Human bone marrow mesenchymal stem cell-derived extracellular vesicles impede the progression of cervical cancer via the miR-144-3p/CEP55 pathway. J Cell Mol Med 25:1867-1883 (2021). PubMed: 33417281